| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AMOTL2 |
| Gene ID: |
51421 |
| Uniprot ID: |
AMOL2_HUMAN |
| Immunogen Region: |
638-837 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 638-837 of human AMOTL2 (NP_001265612.1). |
| Immunogen Sequence: |
RQFAMDAAATAAAQRDTTLI RHSPQPSPSSSFNEGLLTGG HRHQEMESRLKVLHAQILEK DAVIKVLQQRSRRDPGKAIQ GSLRPAKSVPSVFAAAAAGT QGWQGLSSSERQTADAPARL TTDRAPTEEPVVTAPPAAHA KHGSRDGSTQTEGPPDSTST CLPPEPDSLLGCSSSQRAAS LDSVATSRVQDLSDMVEILI |
| Post Translational Modifications | Monoubiquitinated at Lys-347 and Lys-408 by Crumbs complex-bound WWP1. De-ubiquitinated at Lys-347 and Lys-408 by USP9X.the interaction may be promoted by cell contact inhibition. Deubiquitination of AMOTL2 negatively regulates Hippo signaling activation. Phosphorylation at Tyr-107 is necessary for efficient binding to SRC and synergistically functioning with SRC to activate the downstream MAPK pathway. |
| Function | Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Plays a role in coupling actin fibers to cell junctions in endothelial cells and is therefore required for correct endothelial cell morphology via facilitating transcellular transmission of mechanical force resulting in endothelial cell elongation. Required for the anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane which facilitates organization of radial actin fiber structure and cellular response to contractile forces. This contributes to maintenance of cell area, size, shape, epithelial sheet organization and trophectoderm cell properties that facilitate blastocyst zona hatching. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. Activates the Hippo signaling pathway in response to cell contact inhibition via interaction with and ubiquitination by Crumbs complex-bound WWP1. Ubiquitinated AMOTL2 then interacts with LATS2 which in turn phosphorylates YAP1, excluding it from the nucleus and localizing it to the cytoplasm and tight junctions, therefore ultimately repressing YAP1-driven transcription of target genes. Acts to inhibit WWTR1/TAZ transcriptional coactivator activity via sequestering WWTR1/TAZ in the cytoplasm and at tight junctions. Regulates the size and protein composition of the podosome cortex and core at myofibril neuromuscular junctions. Selectively promotes FGF-induced MAPK activation through SRC. May play a role in the polarity, proliferation and migration of endothelial cells. |
| Protein Name | Angiomotin-Like Protein 2Leman Coiled-Coil ProteinLccp |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | Recycling EndosomeCytoplasmCell ProjectionPodosomeCell Junction |
| Alternative Antibody Names | Anti-Angiomotin-Like Protein 2 antibodyAnti-Leman Coiled-Coil Protein antibodyAnti-Lccp antibodyAnti-AMOTL2 antibodyAnti-KIAA0989 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
