Anti-AMOT antibody (1000-1084) (STJ11104583)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Angiomotin (1000-1084) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AMOT |
| Gene ID | 154796 |
| Uniprot ID | AMOT_HUMAN |
| Immunogen Region | 1000-1084 |
| Immunogen Sequence | QTQAPTSAPAVAPTPAPTPT PAVAQAEVPASPATGPGPHR LSIPSLTCNPDKTDGPVFHS NTLERKTPIQILGQEPDAEM VEYLI |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1000-1084 of human AMOT (NP_001106962.1). |
Additional Info
| Tissue Specificity | Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta. |
| Post Translational Modifications | Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation. |
| Function | Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions. |
| Protein Name | Angiomotin |
| Database Links | Reactome: R-HSA-2028269 Q4VCS5-1Reactome: R-HSA-9762292 Q4VCS5-2 |
| Cellular Localisation | Cell JunctionTight JunctionLocalized On The Cell SurfaceMay Act As A Transmembrane Protein |
| Alternative Antibody Names | Anti-Angiomotin antibodyAnti-AMOT antibodyAnti-KIAA1071 antibody |
Information sourced from Uniprot.org