• Western blot analysis of various lysates, using ALDH7A1 rabbit polyclonal antibody (STJ111351) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Negative control (NC) : K-562. Exposure time: 75s.
  • Immunohistochemistry analysis of ALDH7A1 in paraffin-embedded human kidney tissue using ALDH7A1 rabbit polyclonal antibody (STJ111351) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ALDH7A1 in paraffin-embedded mouse colon tissue using ALDH7A1 rabbit polyclonal antibody (STJ111351) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ALDH7A1 in paraffin-embedded rat heart tissue using ALDH7A1 rabbit polyclonal antibody (STJ111351) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of HepG2 cells using ALDH7A1 rabbit polyclonal antibody (STJ111351) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDH7A1 antibody (430-539) (STJ111351)

SKU:
STJ111351

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDH7A1 (430-539) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH7A1
Gene ID: 501
Uniprot ID: AL7A1_HUMAN
Immunogen Region: 430-539
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-539 of human ALDH7A1 (NP_001173.2).
Immunogen Sequence: APILYVFKFKNEEEVFAWNN EVKQGLSSSIFTKDLGRIFR WLGPKGSDCGIVNVNIPTSG AEIGGAFGGEKHTGGGRESG SDAWKQYMRRSTCTINYSKD LPLAQGIKFQ
Tissue Specificity Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in adult peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue.
Function Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.
Protein Name Alpha-Aminoadipic Semialdehyde Dehydrogenase
Alpha-Aasa Dehydrogenase
Aldehyde Dehydrogenase Family 7 Member A1
Antiquitin-1
Betaine Aldehyde Dehydrogenase
Delta1-Piperideine-6-Carboxylate Dehydrogenase
P6c Dehydrogenase
Database Links Reactome: R-HSA-6798163
Reactome: R-HSA-71064
Cellular Localisation Isoform 2: Cytoplasm
Cytosol
Nucleus
Isoform 1: Mitochondrion
Alternative Antibody Names Anti-Alpha-Aminoadipic Semialdehyde Dehydrogenase antibody
Anti-Alpha-Aasa Dehydrogenase antibody
Anti-Aldehyde Dehydrogenase Family 7 Member A1 antibody
Anti-Antiquitin-1 antibody
Anti-Betaine Aldehyde Dehydrogenase antibody
Anti-Delta1-Piperideine-6-Carboxylate Dehydrogenase antibody
Anti-P6c Dehydrogenase antibody
Anti-ALDH7A1 antibody
Anti-ATQ1 antibody

Information sourced from Uniprot.org

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