• Immunofluorescence analysis of U2OS cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ALDH2 antibody (STJ22581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ALDH2 antibody (321-480) (STJ22581)

SKU:
STJ22581

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH2
Gene ID: 217
Uniprot ID: ALDH2_HUMAN
Immunogen Region: 321-480
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 321-480 of human ALDH2 (NP_000681.2).
Immunogen Sequence: AGSRTFVQEDIYDEFVERSV ARAKSRVVGNPFDSKTEQGP QVDETQFKKILGYINTGKQE GAKLLCGGGIAADRGYFIQP TVFGDVQDGMTIAKEEIFGP VMQILKFKTIEEVVGRANNS TYGLAAAVFTKDLDKANYLS QALQAGTVWVNCYDVFGAQS
Post Translational Modifications In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1.
Function Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
Protein Name Aldehyde Dehydrogenase - Mitochondrial
Aldh Class 2
Aldh-E2
Aldhi
Database Links Reactome: R-HSA-380612
Reactome: R-HSA-445355
Reactome: R-HSA-71384
Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Aldehyde Dehydrogenase - Mitochondrial antibody
Anti-Aldh Class 2 antibody
Anti-Aldh-E2 antibody
Anti-Aldhi antibody
Anti-ALDH2 antibody
Anti-ALDM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance