• Immunofluorescence analysis of U2OS cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ALDH2 antibody (STJ22581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ALDH2 antibody (321-480) (STJ22581)

SKU:
STJ22581

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDH2 (321-480) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH2
Gene ID: 217
Uniprot ID: ALDH2_HUMAN
Immunogen Region: 321-480
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 321-480 of human ALDH2 (NP_000681.2).
Immunogen Sequence: AGSRTFVQEDIYDEFVERSV ARAKSRVVGNPFDSKTEQGP QVDETQFKKILGYINTGKQE GAKLLCGGGIAADRGYFIQP TVFGDVQDGMTIAKEEIFGP VMQILKFKTIEEVVGRANNS TYGLAAAVFTKDLDKANYLS QALQAGTVWVNCYDVFGAQS
Function Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
Protein Name Aldehyde Dehydrogenase - Mitochondrial
Aldh Class 2
Aldh-E2
Aldhi
Database Links Reactome: R-HSA-380612
Reactome: R-HSA-445355
Reactome: R-HSA-71384
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Aldehyde Dehydrogenase - Mitochondrial antibody
Anti-Aldh Class 2 antibody
Anti-Aldh-E2 antibody
Anti-Aldhi antibody
Anti-ALDH2 antibody
Anti-ALDM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance