• Western blot analysis of extracts of various cell lines, using ADAM28 Rabbit polyclonal antibody (STJ117049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADAM28 antibody (199-440) (STJ117049)

SKU:
STJ117049

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAM28 (199-440) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAM28
Gene ID: 10863
Uniprot ID: ADA28_HUMAN
Immunogen Region: 199-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 199-440 of human ADAM28 (NP_055080.2).
Immunogen Sequence: VQEHEKYIEYYLVLDNGEFK RYNENQDEIRKRVFEMANYV NMLYKKLNTHVALVGMEIWT DKDKIKITPNASFTLENFSK WRGSVLSRRKRHDIAQLITA TELAGTTVGLAFMSTMCSPY SVGVVQDHSDNLLRVAGTMA HEMGHNFGMFHDDYSCKCPS TICVMDKALSFYIPTDFSSC SRLSYDKFFEDKLSNCLFNA PLPTDIISTPICGNQLVEMG EDCDCGTSEECTNICCDAK
Tissue Specificity Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.
Post Translational Modifications Pro-domain removal and maturation may be, at least in part, autocatalytic.
Function May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation.
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 28
Adam 28
Epididymal Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein Ii
Emdc Ii
Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein L
Mdc-L
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 28 antibody
Anti-Adam 28 antibody
Anti-Epididymal Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein Ii antibody
Anti-Emdc Ii antibody
Anti-Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein L antibody
Anti-Mdc-L antibody
Anti-ADAM28 antibody
Anti-ADAM23 antibody
Anti-MDCL antibody

Information sourced from Uniprot.org

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