• Immunohistochemistry analysis of ACSL3 in paraffin-embedded mouse heart using ACSL3 Rabbit polyclonal antibody (STJ113279) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ACSL3 in paraffin-embedded rat heart using ACSL3 Rabbit polyclonal antibody (STJ113279) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ACSL3 in paraffin-embedded human stomach using ACSL3 Rabbit polyclonal antibody (STJ113279) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ACSL3 in paraffin-embedded human mammary cancer using ACSL3 Rabbit polyclonal antibody (STJ113279) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ACSL3 Rabbit polyclonal antibody (STJ113279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACSL3 antibody (652-672) (STJ113279)

SKU:
STJ113279

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACSL3 (652-672) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSL3
Gene ID: 2181
Uniprot ID: ACSL3_HUMAN
Immunogen Region: 652-672
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 652-672 of human ACSL3 (NP_004448.2).
Immunogen Sequence: CEMENEVLKVLSEAAISASL E
Function Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity.
Protein Name Fatty Acid Coa Ligase Acsl3
Arachidonate--Coa Ligase
Long-Chain Acyl-Coa Synthetase 3
Lacs 3
Long-Chain-Fatty-Acid--Coa Ligase 3
Medium-Chain Acyl-Coa Ligase Acsl3
Database Links Reactome: R-HSA-434313
Reactome: R-HSA-75876
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iii Membrane Protein
Peroxisome Membrane
Microsome Membrane
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Fatty Acid Coa Ligase Acsl3 antibody
Anti-Arachidonate--Coa Ligase antibody
Anti-Long-Chain Acyl-Coa Synthetase 3 antibody
Anti-Lacs 3 antibody
Anti-Long-Chain-Fatty-Acid--Coa Ligase 3 antibody
Anti-Medium-Chain Acyl-Coa Ligase Acsl3 antibody
Anti-ACSL3 antibody
Anti-ACS3 antibody
Anti-FACL3 antibody
Anti-LACS3 antibody

Information sourced from Uniprot.org

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