| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ACHE (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at roo |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ACHE |
| Gene ID: | 43 |
| Uniprot ID: | ACES_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_000656.1; NP_001269378.1 |
| Specificity: | This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form). |
| Immunogen Sequence: | QFDHYSKQDRCSDL |
| Function | Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis. |
| Protein Name | AcetylcholinesteraseAche |
| Database Links | Reactome: R-HSA-112311Reactome: R-HSA-1483191Reactome: R-HSA-422085 |
| Cellular Localisation | SynapseSecretedCell MembranePeripheral Membrane ProteinIsoform T: NucleusOnly Observed In Apoptotic NucleiIsoform H: Cell MembraneLipid-AnchorGpi-AnchorExtracellular Side |
| Alternative Antibody Names | Anti-Acetylcholinesterase antibodyAnti-Ache antibodyAnti-ACHE antibody |
Information sourced from Uniprot.org
