• Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody (STJ118067) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody (STJ118067) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody (STJ118067) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody (STJ118067) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody (STJ118067) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody (STJ118067) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (STJ118067) at 1:1000 dilution.HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-Acetyl-Histone H2B-K12 antibody (STJ118067)

SKU:
STJ118067
Current Stock:
Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against Histone H2B.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Uniprot ID: H2B2E_HUMAN
Gene ID: 8349
Gene Symbol: H2BC21
Immunogen: A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Database Links Reactome: R-HSA-110328
Cellular Localisation Nucleus
Chromosome
Protein Name Histone H2b Type 2-E
H2b-Clustered Histone 21
Histone H2b-Gl105
Histone H2b.q
H2b/Q
Alternative Names Anti-Histone H2b Type 2-E antibody
Anti-H2b-Clustered Histone 21 antibody
Anti-Histone H2b-Gl105 antibody
Anti-Histone H2b.q antibody
Anti-H2b/Q antibody
Anti-H2BC21 antibody
Anti-H2BFQ antibody
Anti-HIST2H2BE antibody
6 months standard