• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H2B-K12 antibody (STJ118067) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours (top left). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours (top left). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours (top left). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded human colon using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Acetyl-Histone H2B-K12 Rabbit polyclonal antibody (STJ118067) at 1:1000 dilution. HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Acetyl-Histone H2B-K12 antibody (STJ118067)

SKU:
STJ118067

$174.83 - $863.33
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H2B-K12 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
Immunogen Sequence: APKKG
Background This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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