| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
TRPM8 Positive Control for STJ503412 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Trpm8 |
| Gene ID: |
171384 |
| Uniprot ID: |
TRPM8_RAT |
| Specificity: |
This is positive control is recommended for use in combination with TRPM8 antibody STJ503412. |
| Tissue Specificity | Expressed in dorsal root and trigeminal ganglia. Specifically expressed in a subset of sensory neurons, including cold-sensitive neurons in trigeminal neurons. |
| Function | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. |
| Peptide Name | Transient Receptor Potential Cation Channel Subfamily M Member 8Cold Menthol Receptor 1 |
| Database Links | Reactome: R-RNO-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinMembrane RaftLocalizes To Membrane Rafts But Is Also Located In The Cell Membrane Outside Of These Regions Where Channel Response To Cold Is Enhanced Compared To Membrane Rafts |
| Alternative Peptide Names | Transient Receptor Potential Cation Channel Subfamily M Member 8 proteinCold Menthol Receptor 1 proteinTrpm8 proteinCmr1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
