TREX1 Positive Control for STJ503388 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:10, 000
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with TREX1 antibody STJ503388.
Additional Info
Tissue Specificity
Detected in thymus, spleen, liver, brain, heart, small intestine and colon.
Post Translational Modifications
Ubiquitinated, but not targeted to proteasomal degradation. Ubiquitination may be important for interaction with UBQLN1.
Function
Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation. Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation. Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Peptide Name
Three-Prime Repair Exonuclease 13'-5' Exonuclease Trex1Deoxyribonuclease IiiDnase Iii
NucleusCytoplasmCytosolEndoplasmic Reticulum MembranePeripheral Membrane ProteinRetained In The Cytoplasm Through The C-Terminal RegionLocalization To The Endoplasmic Reticulum Membrane Is Required To Direct Trex1 To Ruptured MicronucleiIn Response To Dna DamageTranslocates To The Nucleus Where It Is Specifically Recruited To Replication FociTranslocation To The Nucleus Also Occurs During Gzma-Mediated Cell Death
Alternative Peptide Names
Three-Prime Repair Exonuclease 1 protein3'-5' Exonuclease Trex1 proteinDeoxyribonuclease Iii proteinDnase Iii proteinTREX1 protein
