TREX1 Positive Control for STJ503388 peptide (STJ505229)

SKU:
STJ505229-5

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Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: TREX1 Positive Control for STJ503388 is synthetically produced from the sequence and is suitable for use in western blot applications.
Formulation: Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range: WB: 1:10, 000
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: TREX1
Gene ID: 11277
Uniprot ID: TREX1_HUMAN
Specificity: This is positive control is recommended for use in combination with TREX1 antibody STJ503388.
Tissue Specificity Detected in thymus, spleen, liver, brain, heart, small intestine and colon.
Post Translational Modifications Ubiquitinated, but not targeted to proteasomal degradation. Ubiquitination may be important for interaction with UBQLN1.
Function Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation. Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation. Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Peptide Name Three-Prime Repair Exonuclease 1
3'-5' Exonuclease Trex1
Deoxyribonuclease Iii
Dnase Iii
Database Links Reactome: R-HSA-3248023
Reactome: R-HSA-3270619
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Retained In The Cytoplasm Through The C-Terminal Region
Localization To The Endoplasmic Reticulum Membrane Is Required To Direct Trex1 To Ruptured Micronuclei
In Response To Dna Damage
Translocates To The Nucleus Where It Is Specifically Recruited To Replication Foci
Translocation To The Nucleus Also Occurs During Gzma-Mediated Cell Death
Alternative Peptide Names Three-Prime Repair Exonuclease 1 protein
3'-5' Exonuclease Trex1 protein
Deoxyribonuclease Iii protein
Dnase Iii protein
TREX1 protein

Information sourced from Uniprot.org

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