THAP1 Positive Control for STJ503247 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with THAP1 antibody STJ503247.
Additional Info
Tissue Specificity
Kidney and intestine.
Post Translational Modifications
O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1. Phosphorylation at Thr-779 increases SLC9A1 activity. Specifically dephosphorylated at Thr-779 by PPP3CA that negatively regulates SLC9A1 activity. Phosphorylation at Ser-648 by AKT1 reduces SLC9A1 binding to CALM1. Palmitoylated.may play a major role in SLC9A1 regulation.
Function
Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry. Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs.
Peptide Name
Sodium/Hydrogen Exchanger 1ApnhNa(+/H(+ Antiporter - Amiloride-SensitiveNa(+/H(+ Exchanger 1Nhe-1Solute Carrier Family 9 Member 1
Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized Basolaterally In Every Epithelial CellExcept In The Choroid Plexus Where Slc9a1 Is Expressed Luminally
Alternative Peptide Names
Sodium/Hydrogen Exchanger 1 proteinApnh proteinNa(+/H(+ Antiporter - Amiloride-Sensitive proteinNa(+/H(+ Exchanger 1 proteinNhe-1 proteinSolute Carrier Family 9 Member 1 proteinSLC9A1 proteinAPNH1 proteinNHE1 protein
