| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
THAP1 Positive Control for STJ503247 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
SLC9A1 |
| Gene ID: |
6548 |
| Uniprot ID: |
SL9A1_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with THAP1 antibody STJ503247. |
| Immunogen: |
Purified protein. |
| Tissue Specificity | Kidney and intestine. |
| Post Translational Modifications | O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1. Phosphorylation at Thr-779 increases SLC9A1 activity. Specifically dephosphorylated at Thr-779 by PPP3CA that negatively regulates SLC9A1 activity. Phosphorylation at Ser-648 by AKT1 reduces SLC9A1 binding to CALM1. Palmitoylated.may play a major role in SLC9A1 regulation. |
| Function | Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry. Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs. |
| Peptide Name | Sodium/Hydrogen Exchanger 1ApnhNa(+/H(+ Antiporter - Amiloride-SensitiveNa(+/H(+ Exchanger 1Nhe-1Solute Carrier Family 9 Member 1 |
| Database Links | Reactome: R-HSA-2160916Reactome: R-HSA-425986 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized Basolaterally In Every Epithelial CellExcept In The Choroid Plexus Where Slc9a1 Is Expressed Luminally |
| Alternative Peptide Names | Sodium/Hydrogen Exchanger 1 proteinApnh proteinNa(+/H(+ Antiporter - Amiloride-Sensitive proteinNa(+/H(+ Exchanger 1 proteinNhe-1 proteinSolute Carrier Family 9 Member 1 proteinSLC9A1 proteinAPNH1 proteinNHE1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
