St John's Laboratory Ltd

Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T14 Rabbit pAb (STJ22058) at 1:500 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CDK1-T14 antibody (STJ22058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (STJ23048). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U-2 OS cells using KAT5/Tip60 Rabbit polyclonal antibody (STJ28062) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KAT5 antibody (182-282) (STJ28062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CDK1 antibody (N-Term) [M226] (STJA0005340)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-CDK1 antibody [SRM14524] (STJ11109254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using CDK1 antibody (STJ114288) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK1 antibody (1-297) (STJ114288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using Phospho-CDK1-Y15 antibody (STJ11105835) at1:2000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-CDK1-Y15 antibody [S5835RM] (STJ11105835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells using Phospho-CDK1-T14 Rabbit monoclonal antibody (STJ11105900) at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CDK1-T14 antibody [S5900RM] (STJ11105900)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-CDK1-T161+CDK2/CDK3-T160-T161+T160 antibody [SRM15146] (STJ11109876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (STJ22061) at 1:400 dilution. HeLa cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of CDK1 in paraffin-embedded mouse brain using CDK1 Rabbit polyclonal antibody (STJ23048) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK1 antibody (198-297) (STJ23048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot anti-Cdk1 antibody detection in human SYF fibroblasts STJA0041112

Anti-Cdk1 antibody (N-Term) [M226] (STJA0041112)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£474.50

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Western blot Cdk1 and phospho-Cdk1 Tyr-15 in SYF fibroblasts with phosphatase treatment STJA0041113

Anti-Phospho-Cdk1-Tyr-15 antibody [M231] (STJA0041113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Cdk1/p34cdc2 antibody [POH-1] (STJ16101079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Cow/Human/Mink/Monkey

£287.50 - £1,324.50

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Anti-Phospho-CDK1-Tyr-15 antibody [M231] (STJA0005341)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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CDC2/CDK1 antibody western blot K562 A549 Jurkat HeLa STJA0032388

Anti-CDK1 antibody [3B9-F8-B12] (STJA0032388)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from A-431 cells, using JAM-A/CD321 Rabbit polyclonal antibody (STJ11105251) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KAT5 antibody (27-242) (STJ11105251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using CDK1 rabbit monoclonal antibody (STJ11101328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK1 antibody (198-297) [S8MR] (STJ11101328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

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Anti-CDK1 antibody (STJA0033706)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ICC/IF/FC

Reactivity :

Human

£187.50 - £281.50

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CDK1 antibody western blot K562 HeLa STJA0034154

Anti-CDK1 antibody [R05-6I8] (STJA0034154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using AKT1/2/3 (Phospho-Tyr315/316/312) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Akt-Tyr315 antibody (281-330 aa) (STJ91195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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SDS-PAGE gel of recombinant human PARP protein under reducing conditions stained with Coomassie blue

Human PARP protein (Recombinant) (No-Tag) (STJP016611)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Rabbit polyclonal antibody (STJ29827) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-PARP1 antibody (81-390) (STJ29827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of human-lung tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Thr308 antibody (STJ90750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Immunofluorescence analysis of rat-liver tissue. 1, Akt (phospho Ser473) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Ser473 antibody (431-480 aa) (STJ90166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Human PARP (Poly ADP Ribose Polymerase) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017671)

Applications :

ELISA

Reactivity :

Human

£404.50 - £562.50

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Anti-Cleaved-PARP-Asp214 antibody [SRM14950] (STJ11109680)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Cleaved-PARP1 antibody western blot K562 cells STJA0034179

Anti-Cleaved-PARP1 antibody [R02-2C6] (STJA0034179)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various cells using Phospho-Akt1/3 (Y437/434) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-Akt1/3-Tyr437/434 antibody (406-455 aa) (STJ90938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PARP1 antibody [SRM15025] (STJ11109755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Cleaved-PARP1 antibody western blot Jurkat HeLa STJA0035710

Anti-Cleaved-PARP1 antibody [R01-4T4] (STJA0035710)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts of Jurkat cells, using PARP1 antibody (STJ11101720) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PARP1 antibody (700-800) [S0MR] (STJ11101720)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts of various cell lines, using PARP1 antibody (STJ114859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PARP1 antibody (662-1014) (STJ114859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £618.50

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Immunofluorescence analysis of human-stomach tissue. 1, Akt Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Akt antibody (281-330 aa) (STJ91550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Akt1/3 polyclonal antibody diluted at 1:1000 STJ91551

Anti-Akt1/3 antibody (406-455 aa) (STJ91551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human PARP (Poly ADP Ribose Polymerase) Sandwich ELISA Kit (STJE0011284)

Applications :

ELISA

Reactivity :

Human

£386.50 - £537.50

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PARP antibody western blot K562 Ramos Jurkat STJA0032804

Anti-PARP1 antibody [7A1-4H9-5E4] (STJA0032804)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Chicken

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using Cleaved PARP1 p25 antibody (STJ11101731) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PARP1 antibody (150-214) [S1MR] (STJ11101731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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PARP1 antibody western blot K562 3T3 HeLa STJA0034689

Anti-PARP1 antibody [R05-8E8] (STJA0034689)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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PARP1 antibody western blot Jurkat HeLa STJA0035714

Anti-PARP1 antibody [R07-3A9] (STJA0035714)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-HMGB1 antibody [DPH11] (STJA0019032)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/FuncS/IF/WB

Reactivity :

Human/Mouse/Rat/Bovine/Dog/Horse/Pig

£195.50 - £1,319.50

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Rat Hmgb1 (High mobility group protein B1) Sandwich ELISA Kit (STJE0005822)

Applications :

ELISA

Reactivity :

Rat

£161.50 - £450.50

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Mouse HMGB1 protein (Recombinant) (C-His) (STJP002772)

Host :

HEK293 cells

£120.50 - £411.50

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Mouse HMGB1 protein (Recombinant) (N-His) (STJP006817)

Host :

Mammalian Cells

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£267.50 - £2,006.50

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HepG2 cells treated with UV using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HMGB1-Lys12 antibody (STJ97227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit polyclonal antibody (STJ11102770) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-HMGB1-K29 antibody (STJ11102770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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