St John's Laboratory Ltd

STJ73296 (1µg/ml) staining HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-KAP1 antibody (Internal) (STJ73296)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot with Anti-MAPK11 Antibody (STJ501684) of 1) Rat cortex, 2) Mouse cerebellum, 3) Mouse lung, 4) HEK cells. Antibody is at 1:500 dilution in buffer.

Anti-MAPK11 antibody (300-350) (STJ501684)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-MAPK11 antibody (300-350) {Biotin} (STJ501685)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SGK1 antibody (40-90) {FITC} (STJ502980)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Rat PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit (STJE0011497)

Applications :

ELISA

Reactivity :

Rat

£432.50 - £602.50

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Human PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017881)

Applications :

ELISA

Reactivity :

Human

£423.50 - £587.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PKC (PTR1190) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 80kDa

Anti-PRKCA antibody (450-550) [PTR1190] (STJA0006229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Bovine

£49.00 - £261.00

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Anti-Recombinant-Phospho-PRKCA/PKC alpha-Ser657 antibody [RM1Q33] (STJA0024017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-PRKCA/PKC alpha-Thr638 antibody [RM1Q34] (STJA0024018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-PRKCA antibody [RM1B40] (STJA0026810)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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SDS-PAGE gel of recombinant human KAP1 protein 3µg under reducing conditions Coomassie blue stain

Human KAP1 protein (Recombinant) (His-Tag) (STJP018428)

Host :

E.coli

Applications :

SDS-PAGE/Denatured

£134.50 - £738.50

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Anti-PKC Alpha antibody (Central Region) [M237] (STJA0041317)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£579.50

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SGK1 antibody immunohistochemistry human brain tissue STJA0032892

Anti-SGK1 antibody [6E4-8G5-6D10] (STJA0032892)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Phospho-CDK2 Tyr15 antibody western blot C6 UV lysates STJA0033906

Anti-Phospho-CDK2-Tyr15 antibody [R05-2C4] (STJA0033906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

£133.50 - £281.50

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Phospho-CDK2 Thr14 antibody western blot HeLa A549 HL-60 U2OS STJA0035056

Anti-Phospho-CDK2-Thr14 antibody [R06-4C1] (STJA0035056)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using KAP1/TRIM28 antibody (STJ11101703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TRIM28 antibody (250-350) [S3MR] (STJ11101703)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer using [KO Validated] CDK2 Rabbit polyclonal antibody (STJ113540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK2 antibody (200-298) (STJ113540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of L929 cells using SGK1 Rabbit polyclonal antibody (STJ25513) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGK1 antibody (172-431) (STJ25513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis using ARK-2 monoclonal antibody against truncated AURKB recombinant protein (1) and SKN-SH cell lysate (2).

Anti-AURKB antibody [13E8D3] (STJ97851)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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STJ70307 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-KAP1/TRIM28 antibody (C-Term) (STJ70307)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC/IHC

Reactivity :

Human/Mouse

£312.50

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Anti-SGK1 antibody (40-90) {Biotin} (STJ502979)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Human THBS1 (Thrombospondin-1) Sandwich ELISA Kit (STJE0003086)

Applications :

ELISA

Reactivity :

Human

£161.50 - £450.50

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Mouse TSP-1 (Thrombospondin-1) Sandwich ELISA Kit (STJE0019907)

Applications :

ELISA

Reactivity :

Mouse

£161.50 - £450.50

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Western blot analysis of various lysates, using PKC alpha Rabbit monoclonal antibody (STJ11105041) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKCA antibody (200-300 aa) [S5041RM] (STJ11105041)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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PP2A alpha/beta antibody western blot C2C12 3T3 mouse brain STJA0011815

Anti-PPP2CA antibody (277-294 aa) [R04-4G4] (STJA0011815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

£133.50 - £281.50

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Anti-PKC alpha antibody [SRM5132] (STJ11106955)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-TRIM28 IP antibody [SAA0249] (STJA0018764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Recombinant-PRKCA antibody [RM1Q36] (STJA0024020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-AURKB antibody [RM2S26] (STJA0026584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Human

£300.50 - £457.50

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Human TRIM28 protein (Recombinant) (N-His) (STJP010092)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Phospho-PKC Alpha-S657/Y658 antibody (STJA0041328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IP

Reactivity :

Human/Rabbit/Rat

£542.50

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Western blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (STJ11101090) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PRKCA-S657/Y658 antibody (STJ11101090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using CDK2 rabbit monoclonal antibody (STJ11101225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK2 antibody (199-298) [S5MR] (STJ11101225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using SIRT6 Rabbit monoclonal antibody (STJ11101906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SIRT6 antibody (256-355) [S6MR] (STJ11101906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK2-Y15 Rabbit mAb (STJ11102594) at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours or treated by Nocodazole (100 ng/mL) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CDK2-Y15 antibody [S4MR] (STJ11102594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic Alpha-Y307 Rabbit mAb (STJ11102616) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-PPP2CA-Y307 antibody [S6MR] (STJ11102616)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells, using Phospho-PKC alpha-S319 Rabbit pAb (STJ117877) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-S319 antibody (STJ117877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PRKCA-Thr638/641 antibody (STJ196382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, using AurB (Phospho-Tyr12) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Tyr12 antibody (1-50 aa) (STJ90920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Mouse PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit (STJE0011496)

Applications :

ELISA

Reactivity :

Mouse

£413.50 - £575.50

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Mouse PKCa (Protein Kinase C Alpha) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017882)

Applications :

ELISA

Reactivity :

Mouse

£432.50 - £602.50

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Mouse SIRT6 (Sirtuin 6) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018567)

Applications :

ELISA

Reactivity :

Mouse

£451.50 - £629.50

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Immunofluorescence analysis of HeLa cells using PKC alpha Rabbit monoclonal antibody (STJ11105054) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKCA antibody [S5054RM] (STJ11105054)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of 293T cells, using Phospho-PKC alpha-T638 antibody (STJ11105853) at 1:7000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-PRKCA-T638 antibody [S5853RM] (STJ11105853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit monoclonal antibody (STJ11105902) at 1:1000 dilution. C6 cells were treated by Nocodazole (10ng/ml). Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CDK2-T160 antibody [S5902RM] (STJ11105902)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£143.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PKC alpha antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PRKCA antibody (658-672 aa) [R05-4E5] (STJA0013069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Anti-Recombinant-Phospho-PRKCA-T638 antibody [RM1Q31] (STJA0024015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-PRKCA-T497 antibody [RM1Q32] (STJA0024016)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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