St John's Laboratory Ltd

Western blot analysis of various lysates using APPL11 Rabbit mAb (STJ11102397) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-APPL1 antibody (350-450) [S7MR] (STJ11102397)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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STJ71081 (0. 5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.

Anti-APPL1 antibody (Internal) (STJ71081)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-WWP1 antibody (N-Term) (STJA0005653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Mouseat TGFB2/TGF-beta-2 protein (Recombinant-Active) (Tag free) (STJP012863)

Host :

Mammalian Cells

Applications :

ELISA/WB

£624.50

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Anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 antibody (C-Term) (STJA0040888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/WB

Reactivity :

Human/Mouse

£540.50

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SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating STJ193468

Anti-NEDD4L antibody (28-78 aa) (STJ193468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Mouse CIDEC protein (Recombinant) (N-His) (STJP011153)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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SDS-PAGE gel of recombinant human Smad3 protein under reducing conditions Coomassie blue stain

Human Smad3 protein (Recombinant) (His-Tag) (STJP016563)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Western blot analysis of extracts of various cell lines, using APPL1 antibody (STJ22658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APPL1 antibody (1-210) (STJ22658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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APPL antibody western blot rat brain C6 3T3 HeLa STJA0034032

Anti-APPL antibody [R05-8D5] (STJA0034032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD3 antibody (100-200) [S2MR] (STJ11101652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates using (STJ11103777) at 1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Smad3-S423/S425 antibody (STJ11103777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Phospho-Smad3 antibody western blot PC-12 lysates STJA0035799

Anti-Phospho-Smad3-Ser423/425 antibody [R02-7Z7] (STJA0035799)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Smad3 antibody immunohistochemistry human skin tissue STJA0032933

Anti-Phospho-Smad3-Ser425 antibody [7H6-4E5-4H2] (STJA0032933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Western blot analysis of mouse-kidney 293T mouse-lung lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-TGFB2 antibody (361-410 aa) (STJ98769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot WWP1 expression in mouse liver and PC3 cells using antibody STJA0041456

Anti-WWP1 antibody (N-Term) (STJA0041456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

£474.50

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Human CIDEC protein (Recombinant) (N-His) (STJP005826)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Mouse Smad3 (Mothers Against Decapentaplegic Homolog 3) Sandwich ELISA Kit (STJE0012231)

Applications :

ELISA

Reactivity :

Mouse

£432.50 - £601.50

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Western blot analysis of various lysates using NEDD4L Rabbit pAb (STJ110385) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NEDD4L antibody (836-955) (STJ110385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western Blot analysis of 1 Hela treated with LPS, 2 Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NEDD4L-Ser342 antibody (STJ196363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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NEDD4-2 antibody western blot K562 HeLa lysates STJA0035442

Anti-NEDD4-2 antibody [R41-8P-2] (STJA0035442)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of Rat skeletal muscle, using WWP1 antibody (STJ117813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WWP1 antibody (1-260) (STJ117813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ73581 (0. 3µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-prepro-transforming growth factor beta-2 antibody (Internal) (STJ73581)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Pig/Cow

£312.50

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Anti-TGFB2/TGF-beta-2 antibody [SAA0444] {FITC} (STJA0028518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Biotinylated Human TGFB2/TGF-beta-2 protein (N-Avi) {Biotin} (STJP012625)

Host :

Mammalian Cells

Applications :

ELISA/WB

£1,967.50

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Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (STJ11102566) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Smad3-S423/S425 antibody [S6MR] (STJ11102566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-Smad3 antibody [SRM14491] (STJ11109221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Recombinant-NEDD4L/Nedd4-2 antibody [RM2L61] (STJA0026025)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Western blot analysis of various cells using TGF Beta 3 Polyclonal Antibody

Anti-TGFB3 antibody (261-310 aa) (STJ95999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SMAD7 antibody (STJ192434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Goat/Sheep

£49.00 - £261.00

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Anti-TGFB3/TGF-beta-3 antibody [SAA0445] {PE} (STJA0031050)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Biotinylated Human TGFB3/TGF-beta-3 protein (N-Avi) {Biotin} (STJP012458)

Host :

Mammalian Cells

Applications :

ELISA/WB

£1,967.50

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Human SMAD7 protein (Recombinant) (N-His) (STJP003216)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-TGFB2/TGF-beta-2 antibody [SAA0444] (STJA0027621)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-TGFB2/TGF-beta-2 antibody [SAA0444] {PE} (STJA0031416)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-TGFB2/TGF-beta-2 antibody [SAA0444] {PerCP} (STJA0030450)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-WWP1 antibody (N-Term) (STJA0010355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-APPL1 antibody [RM2K81] (STJA0025946)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-TGFB3/TGF-beta-3 antibody [SAA0445] (STJA0027622)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-TGFB3/TGF-beta-3 antibody [SAA0445] {FITC} (STJA0028152)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Human TGFB2/TGF-beta-2 protein (Recombinant-Active) (No tag) (STJP012039)

Host :

Mammalian Cells

Applications :

ELISA/WB

£503.50

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Biotinylated Human Latent TGF-beta-2 protein (N-8*His-Avi) {Biotin} (STJP012624)

Host :

Mammalian Cells

Applications :

ELISA/WB

£1,967.50

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Anti-Phospho-NEDD4L-Ser448 antibody (STJ196364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using CIDEC Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CIDEC antibody (189-238 aa) (STJ92292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Smad3 antibody western blot 293T Jurkat rat mouse heart STJA0032967

Anti-Smad3 antibody [4H4-10A10-7B4] (STJA0032967)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Smad3 antibody western blot 3T3 C6 STJA0034867

Anti-Smad3 antibody [R02-1I2] (STJA0034867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Hamster

£133.50 - £281.50

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