Anti-prepro-transforming growth factor beta-2 antibody (Internal) (STJ73581)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-prepro-transforming growth factor beta-2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in Human Breast cancer lysates (calculated MW of 50.6kDa according to NP_001129071.1;). This molecular weight is routinely observed by other sources. Recomme |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TGFB2 |
| Gene ID | 7042 |
| Uniprot ID | TGFB2_HUMAN |
| Accession Number | NP_001129071.1; NP_003229.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | STYTSGDQKTIKSTR |
| Specificity | This antibody is expected to recognize the part representing the latency-associated peptide. |
Additional Info
| Function | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Protein Name | Transforming Growth Factor Beta-2 ProproteinCeterminGlioblastoma-Derived T-Cell Suppressor FactorG-Tsf Cleaved Into - Latency-Associated PeptideLap - Transforming Growth Factor Beta-2Tgf-Beta-2 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-2129379Reactome: R-HSA-2173789Reactome: R-HSA-3000178Reactome: R-HSA-9839389 |
| Cellular Localisation | Latency-Associated Peptide: SecretedExtracellular SpaceExtracellular MatrixTransforming Growth Factor Beta-2: Secreted |
| Alternative Antibody Names | Anti-Transforming Growth Factor Beta-2 Proprotein antibodyAnti-Cetermin antibodyAnti-Glioblastoma-Derived T-Cell Suppressor Factor antibodyAnti-G-Tsf Cleaved Into - Latency-Associated Peptide antibodyAnti-Lap - Transforming Growth Factor Beta-2 antibodyAnti-Tgf-Beta-2 antibodyAnti-TGFB2 antibody |
Information sourced from Uniprot.org