St John's Laboratory Ltd

Anti-Phospho-SRC-Ser17 antibody (STJ196435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1:1000 STJ90446

Anti-Phospho-SRC-Tyr419 antibody (STJ90446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Src (PTR2316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HUVEC Lane 3: A431Predicted band size: 60kDaObserved band size: 60kDa

Anti-SRC antibody (400-536 aa) [PTR2316] (STJ193243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Src antibody (200-300) {Biotin} (STJ502689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-Src antibody (200-300) {FITC} (STJ502690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Western blot analysis of A-431, using Phospho-Src-Y529 antibody (STJ11105854) at 1:10000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-SRC-Y529 antibody [S5854RM] (STJ11105854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Human SRC protein (Recombinant) (N-His) (STJP004052)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis c-Src phosphorylation in SYF cells using antibody STJA0041379

Anti-Phospho-c-Src-Tyr-530 antibody (C-Term) [M261] (STJA0041379)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot c-Src phosphorylation in mouse SYF cells with pervanadate treatment STJA0041394

Anti-Phospho-c-Src-Tyr-215 antibody (STJA0041394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Phospho-Src antibody western blot rat lung STJA0035099

Anti-Phospho-Src-Tyr530 antibody [R03-8H8] (STJA0035099)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Rat

£133.50 - £281.50

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Western blot analysis of lysates from 293T cells, using Phospho-Src-S17 Rabbit pAb (STJ113487) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SRC-S17 antibody (STJ113487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit pAb (STJ22407) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SRC-Y529 antibody (STJ22407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-SRC-Tyr529 antibody (487-536 aa) (STJ90238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-Phospho-Src antibody (400-500) {Biotin} (STJ502691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-SRC antibody (200-300) (STJ503200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Immunohistochemistry of rat tissue with Anti-SRC primary antibody (STJ503201) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SRC antibody (400-500) (STJ503201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-SRC antibody (200-300) {FITC} (STJ503203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Phospho-SRC-Tyr-215 antibody (STJA0005602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (STJ25533) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT1 antibody (229-246) (STJ25533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SIRT1-Ser47 antibody (STJ90648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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STJ70991 (1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-SIRT1 antibody (Internal) (STJ70991)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-SIRT1 antibody (25-75) {FITC} (STJ503031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Chicken/Human/Monkey/Mouse/Rat

£497.50

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Phospho-SIRT1 antibody western blot HEK293 lysates STJA0036024

Anti-Phospho-SIRT1-Ser47 antibody [R04-7M2] (STJA0036024)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Phospho-SIRT1 antibody western blot 293T cells STJA0036136

Anti-Phospho-SIRT1-Thr530 antibody [R06-2U8] (STJA0036136)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Human SIRT1 protein (Recombinant) (N-His) (STJP005836)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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SDS-PAGE gel of recombinant human SIRT1 protein 3µg under reducing conditions stained with Coomassie blue

Human Sirtuin 1/SIRT1 protein (Recombinant) (His-Tag) (STJP018722)

Host :

E.coli

Applications :

SDS-PAGE/Denatured

£134.50 - £738.50

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SIRT1-Ser27 antibody (STJ196424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis using SIRT1 monoclonal antibody against MCF-7 (1) , Jurkat (2) , HeLa (3) , HEK293 (4) and A549 (5) cell lysate.

Anti-SIRT1 antibody [1F3] (STJ98380)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Monkey

£149.00 - £360.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SIRT1 antibody (STJA0009872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-SIRT1 antibody [RM2J68] (STJA0025834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-SIRT1 antibody [5B2] (STJA0032634)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Immunohistochemistry analysis of paraffin-embedded human colon using SIRT1 Rabbit polyclonal antibody (STJ113751) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SIRT1 antibody (448-747) (STJ113751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SIRT1 antibody (448-747) (STJ119437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, SIRT1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SIRT1 antibody (13-62 aa) (STJ95667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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STJ72538 (0. 05µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Sirtuin 1/SIRT1 antibody (577-590) (STJ72538)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse

£312.50

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SIRT1 Blocking Peptide for STJ503029 peptide (STJ504932)

Applications :

Immunodepletion/Immunocompetition

£260.50

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SIRT1 Antibody Positve Control Positive Control peptide (STJ505152)

Applications :

WB

£308.50

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Human SIRT1 (SIRT1) Antibody Pair (STJAP00957)

Host :

Sheep/Sheep

Applications :

ELISA

Reactivity :

Human

£807.50

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Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb (STJ11102569) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-SIRT1-S47 antibody [S9MR] (STJ11102569)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry of rat tissue with Anti-SIRT1 primary antibody (STJ503029) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SIRT1 antibody (25-75) (STJ503029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Chicken/Human/Monkey/Mouse/Rat

£400.50

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Anti-SIRT1 antibody (25-75) {Biotin} (STJ503030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Chicken/Human/Monkey/Mouse/Rat

£497.50

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Anti-Phospho-SIRT1-Ser549 antibody (STJA0009874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-SIRT1 antibody [PT0158R] (STJA0039739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Rat

£150.00 - £540.00

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SIRT1 antibody western blot HeLa Jurkat Ramos A549 CEM STJA0032465

Anti-SIRT1 antibody [2G10-F4-D9] (STJA0032465)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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SIRT1 antibody western blot K562 C6 3T3 HeLa STJA0034857

Anti-SIRT1 antibody [R06-4K9] (STJA0034857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot detection of SIRT1 in Hela, Jurkat, Ramos, A549 and CEM cell lysates using SIRT1 mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.

Anti-SIRT1 antibody [2G10-F4-D9] (STJ99238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£149.00 - £360.00

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Human SIRT1 (NAD-dependent protein deacetylase sirtuin-1) CLIA Kit for Quantitative Detection

Applications :

CLIA

Reactivity :

Human

£177.50 - £448.50

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Human SIRT1 (NAD-dependent protein deacetylase sirtuin-1) Sandwich ELISA Kit (STJE0003049)

Applications :

ELISA

Reactivity :

Human

£161.50 - £450.50

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