• Western blot analysis of extracts of various cell lines, using Phospho-Src-Y529 polyclonal antibody (STJ22407) at 1:1000 dilution. Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour or treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of A-431 cells, using Phospho-Src-Y529 antibody (STJ22407) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-SRC-Y529 antibody (STJ22407)

SKU:
STJ22407

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-SRC-Y529 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRC
Gene ID: 6714
Uniprot ID: SRC_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y529 of human Src (NP_005408.1).
Immunogen Sequence: EPQYQ
Tissue Specificity Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. Isoform 1: Expressed in spleen and liver. Isoform 2: Expressed in brain. Isoform 3: Expressed in brain.
Post Translational Modifications Myristoylated at Gly-2, and this is essential for targeting to membranes. Dephosphorylated at Tyr-530 by PTPRJ. Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation. CDK5-mediated phosphorylation at Ser-75 targets SRC to ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. Phosphorylated by PTK2/FAK1.this enhances kinase activity. Phosphorylated by PTK2B/PYK2.this enhances kinase activity. Upon activation of IL6ST by IL6, Tyr-419 is phosphorylated and Tyr-530 dephosphorylated. Isoform 1: Displays reduced levels of autophosphorylation at Tyr-419 compared to isoforms 2 and 3. Isoform 2: Displays enhanced levels of autophosphorylation at Tyr-419 compared to isoform 1. Isoform 3: Displays enhanced levels of autophosphorylation at Tyr-419 compared to isoform 1. Shows reduced phosphorylation at Tyr-527 compared to isoforms 1 and 2. S-nitrosylation is important for activation of its kinase activity. Ubiquitinated in response to CDK5-mediated phosphorylation. Ubiquitination mediated by CBLC requires SRC autophosphorylation at Tyr-419 and may lead to lysosomal degradation.
Function Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN) (Probable). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN). In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors. Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1. Plays a role in EGF-mediated calcium-activated chloride channel activation. Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a critical role in the stimulation of the CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor (Probable). Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus. Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates DDR2 on tyrosine residues, thereby promoting its subsequent autophosphorylation. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances RIGI-elicited antiviral signaling. Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373' and 'Tyr-376'. Phosphorylates BCAR1 at 'Tyr-128'. Phosphorylates CBLC at multiple tyrosine residues, phosphorylation at 'Tyr-341' activates CBLC E3 activity. Phosphorylates synaptic vesicle protein synaptophysin (SYP). Involved in anchorage-independent cell growth. Required for podosome formation. Mediates IL6 signaling by activating YAP1-NOTCH pathway to induce inflammation-induced epithelial regeneration. Phosphorylates OTUB1, promoting deubiquitination of RPTOR. Isoform 1: Non-receptor protein tyrosine kinase which phosphorylates synaptophysin with high affinity. Isoform 2: Non-receptor protein tyrosine kinase which shows higher basal kinase activity than isoform 1, possibly due to weakened intramolecular interactions which enhance autophosphorylation of Tyr-419 and subsequent activation. The SH3 domain shows reduced affinity with the linker sequence between the SH2 and kinase domains which may account for the increased basal activity. Displays altered substrate specificity compared to isoform 1, showing weak affinity for synaptophysin and for peptide substrates containing class I or class II SH3 domain-binding motifs. Plays a role in L1CAM-mediated neurite elongation, possibly by acting downstream of L1CAM to drive cytoskeletal rearrangements involved in neurite outgrowth. Isoform 3: Non-receptor protein tyrosine kinase which shows higher basal kinase activity than isoform 1, possibly due to weakened intramolecular interactions which enhance autophosphorylation of Tyr-419 and subsequent activation. The SH3 domain shows reduced affinity with the linker sequence between the SH2 and kinase domains which may account for the increased basal activity. Displays altered substrate specificity compared to isoform 1, showing weak affinity for synaptophysin and for peptide substrates containing class I or class II SH3 domain-binding motifs. Plays a role in neurite elongation.
Protein Name Proto-Oncogene Tyrosine-Protein Kinase Src
Proto-Oncogene C-Src
Pp60c-Src
P60-Src
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1251985
Reactome: R-HSA-1253288
Reactome: R-HSA-1257604
Reactome: R-HSA-1295596 P12931-1
Reactome: R-HSA-1433557 P12931-1
Reactome: R-HSA-1433559 P12931-1
Reactome: R-HSA-171007 P12931-1
Reactome: R-HSA-177929 P12931-1
Reactome: R-HSA-180292
Reactome: R-HSA-186763
Reactome: R-HSA-191650 P12931-2
Reactome: R-HSA-201556 P12931-1
Reactome: R-HSA-2029481 P12931-1
Reactome: R-HSA-210990 P12931-1
Reactome: R-HSA-2219530
Reactome: R-HSA-2682334 P12931-1
Reactome: R-HSA-354192
Reactome: R-HSA-354194
Reactome: R-HSA-372708
Reactome: R-HSA-375165 P12931-1
Reactome: R-HSA-389356 P12931-1
Reactome: R-HSA-389513 P12931-1
Reactome: R-HSA-391160 P12931-1
Reactome: R-HSA-3928662 P12931-1
Reactome: R-HSA-3928663 P12931-1
Reactome: R-HSA-3928664 P12931-1
Reactome: R-HSA-3928665 P12931-1
Reactome: R-HSA-418555
Reactome: R-HSA-418592 P12931-1
Reactome: R-HSA-418594
Reactome: R-HSA-418885
Reactome: R-HSA-418886
Reactome: R-HSA-428542
Reactome: R-HSA-430116 P12931-1
Reactome: R-HSA-437239 P12931-1
Reactome: R-HSA-4420097 P12931-1
Reactome: R-HSA-456926 P12931-1
Reactome: R-HSA-5218921 P12931-1
Reactome: R-HSA-5607764 P12931-1
Reactome: R-HSA-5663220 P12931-1
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-6811558
Reactome: R-HSA-69231 P12931-1
Reactome: R-HSA-8853659 P12931-1
Reactome: R-HSA-8874081
Reactome: R-HSA-8876493
Reactome: R-HSA-8934593
Reactome: R-HSA-8934903 P12931-1
Reactome: R-HSA-8940973
Reactome: R-HSA-8941858
Reactome: R-HSA-9009391
Reactome: R-HSA-9013420
Reactome: R-HSA-9032500
Reactome: R-HSA-9603381
Reactome: R-HSA-9620244
Reactome: R-HSA-9634597
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9664323 P12931-1
Reactome: R-HSA-9664422 P12931-1
Reactome: R-HSA-9670439 P12931-1
Reactome: R-HSA-9680350
Cellular Localisation Cell Membrane
Lipid-Anchor
Mitochondrion Inner Membrane
Nucleus
Cytoplasm
Cytoskeleton
Perinuclear Region
Cell Junction
Focal Adhesion
Localizes To Focal Adhesion Sites Following Integrin Engagement
Localization To Focal Adhesion Sites Requires Myristoylation And The Sh3 Domain
Colocalizes With Pdlim4 At The Perinuclear Region
But Not At Focal Adhesions
Alternative Antibody Names Anti-Proto-Oncogene Tyrosine-Protein Kinase Src antibody
Anti-Proto-Oncogene C-Src antibody
Anti-Pp60c-Src antibody
Anti-P60-Src antibody
Anti-SRC antibody
Anti-SRC1 antibody

Information sourced from Uniprot.org

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