Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of NIH/3T3, using Phospho-EIF4EBP1-S65 antibody (STJ11105846) at 1:20000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-S65 antibody [S5846RM] (STJ11105846)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 4E-BP1 (Phospho-Ser64) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EIF4EBP1-Ser65 antibody (30-79 aa) (STJ90778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from MDA-MB-435 cells, using Phospho-eIF4EBP1-T36 Rabbit pAb (STJ22121). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-EIF4EBP1-T36 antibody (STJ22121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37 Rabbit monoclonal antibody (STJ11105858) at 1:1000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-EIF4EBP1-T37 antibody [S5858RM] (STJ11105858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

Choose Options

Western blot analysis of various lysates using Phospho-eIF4EBP1-T37/46 Rabbit pAb (STJ22117) at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-EIF4EBP1-T37/46 antibody (STJ22117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37/T46 Rabbit monoclonal antibody (STJ11105857) at 1:7000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-T37/T46 antibody [S5857RM] (STJ11105857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

Choose Options

Western blot analysis of lysates from HeLa cells using Phospho-eIF4EBP1-T45 Rabbit pAb (STJ22120). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-EIF4EBP1-T45 antibody (STJ22120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£126.50 - £618.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-eIF4EBP1-T70 Rabbit pAb (STJ22118) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-EIF4EBP1-T70 antibody (STJ22118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of extracts of NIH/3T3, using Phospho-eIF4EBP1-T70 antibody (STJ11105824) at1:5000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-T70 antibody [S5824RM] (STJ11105824)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Western Blot (WB): Gao, Hai-na, et al. "Leucine and histidine independently regulate milk protein synthesis in bovine STJ90161

Anti-Phospho-EIF4EBP1-Thr37 antibody (4-53 aa) (STJ90161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Chicken/Monkey/Sheep/Fish/Guinea Pig/Cow

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EIF4EBP1-Thr46 antibody (13-62 aa) (STJ90162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 4E-BP1 (Phospho-Thr70) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EIF4EBP1-Thr70 antibody (36-85 aa) (STJ91237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-EIF4G1-S1108 Rabbit pAb (STJ117894) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-EIF4G1-S1108 antibody (STJ117894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Anti-Phospho-EIF4G1-Ser1108 antibody (STJ196298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eIF4G (Phospho-Ser1108) Antibody

Anti-Phospho-EIF4G1-Ser1148 antibody (1074-1123 aa) (STJ91130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from Jurkat cells, using Phospho-ELK1-S383 Rabbit pAb (STJ22123) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ELK1-S383 antibody (STJ22123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Ser383) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Ser383 antibody (351-400 aa) (STJ90255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot phospho-Elk-1 Ser383 immunolabeling with phosphopeptide competition STJA0003626

Anti-Phospho-ELK1-Ser383 antibody (STJA0003626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Canine/Human/Non-Human Primates/Zebrafish

£488.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Ser389) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Ser389 antibody (356-405 aa) (STJ90256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Thr417) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Thr417 antibody (379-428 aa) (STJ90257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot eNOS phosphorylation at Ser-632 and Ser-1177 in endothelial cells STJA0041299

Anti-Phospho-eNOS-Ser-1177 antibody (STJA0041299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

Choose Options

Western blot anti-eNOS Tyr-657 phospho-specific antibody in endothelial cells STJA0041298

Anti-Phospho-eNOS/nNOS-Tyr-657 antibody (STJA0041298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

Choose Options

Anti-Phospho-EP300-Ser1834 antibody (STJA0009836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Anti-Phospho-EP300-Ser1834 antibody (STJA0009894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using p300 (Phospho-Ser89) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EP300-Ser89 antibody (55-104 aa) (STJ91120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from MDA-MB-231 cells, using Phospho-EPHA2-S897 Rabbit pAb (STJ11101120) at 1:400 dilution. MDA-MB-231 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-EPHA2-S897 antibody (STJ11101120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Anti-Phospho-EPHA2-S897 antibody [SRM13704] (STJ11108434)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-EPHA2-Ser897 antibody (STJ196300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using Phospho-EphA2 (Y588) Polyclonal Antibody

Anti-Phospho-EPHA2-Tyr588 antibody (STJ90258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Anti-Phospho-EPHA2-Tyr772 antibody (STJ196301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Anti-Phospho-EPHA2-Tyr930 antibody (STJA0010030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from SH-SY5Y cells, using Phospho-EPHA2-Y594 Rabbit pAb (STJ117916) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/mL) for 5 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-EPHA2-Y594 antibody (STJ117916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of K562 cells using Phospho-EpoR (Y368) Polyclonal Antibody

Anti-Phospho-EPOR-Tyr368 antibody (341-390 aa) (STJ90712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of extracts from K562 cells, using Phospho-EpoR (Y426) Polyclonal Antibody.

Anti-Phospho-EPOR-Tyr426 antibody (STJ91365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody

Anti-Phospho-EPS15-Tyr849 antibody (STJ90629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Lane 2 & 4) Western blot with Anti-Phospho-ErbB 2 Antibody (STJ502480) on Herceptin-2.

Anti-Phospho-ErbB 2 antibody (1100-1150) (STJ502480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

Choose Options

Anti-Phospho-ErbB 2 antibody (1100-1150) {Biotin} (STJ502481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

Choose Options

Anti-Phospho-ErbB 2 antibody (1100-1150) {FITC} (STJ502482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

Choose Options

Phospho-ErbB2 antibody western blot SKBR3 lysates STJA0035700

Anti-Phospho-ErbB 2-Tyr1221/Tyr1222 antibody [R87-5Y-7] (STJA0035700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

Choose Options

phospho-ErbB2 EGFR antibody western blot HeLa STJA0033910

Anti-Phospho-ErbB 2/EGFR-Tyr1248/Tyr1173 antibody [R03-6A8] (STJA0033910)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

Choose Options

Immunofluorescence analysis of HUVEC cells, using HER2 (Phospho-Thr686) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ERBB2-Thr686 antibody (661-710 aa) (STJ91335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-ERBB2-Thr686 antibody (STJA0009829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of K562 using p-Neu (Y1112) antibody. Antibody was diluted at 1:500

Anti-Phospho-ERBB2-Tyr1112 antibody (1081-1130 aa) (STJ90573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ERBB2-Tyr1139 antibody (STJ193220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Phospho-ErbB2 antibody western blot A431 cells STJA0035681

Anti-Phospho-ErbB2-Tyr1139 antibody [R78-8J-4] (STJA0035681)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-ERBB2-Tyr1196 antibody (STJ196320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Polyclonal Antibody

Anti-Phospho-ERBB2-Tyr1221/Y1222 antibody (1191-1240 aa) (STJ90337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-ERBB2-Tyr1221/Y1222 antibody (STJ91025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options