Programmed Cell Death | Research Area | St John's Laboratory

Western blot analysis of various lysates, using OMA1 Rabbit polyclonal antibody (STJ11105230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-OMA1 antibody (421-520) (STJ11105230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-OMA1 antibody (STJA0006850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates, using OPA1 Rabbit pAb (STJ111875) at 1:7000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-OPA1 antibody (200-960) (STJ111875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-OPA1 antibody (N-Term) (STJ13101547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

Choose Options

Sirt6 deficiency contributes to mitochondrial fission and oxidative damage in podocytes via ROCK1-Drp1 signalling pathway CELL PROLIFERATION Guohua Ding WB Human

Anti-OPA1 antibody (STJ193080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of P40 at dilution of 1:1000

Anti-p40-DeltaNp63 antibody [SM5075] (STJA0005075)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

Choose Options

Anti-P53 antibody [BP53+12] (STJ16100968)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/IF

Reactivity :

Human

£521.50

Choose Options

Anti-p53 antibody [BP53-12] (STJ16100743)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Chicken/Dog/Hamster/Human/Monkey

£287.50 - £1,324.50

Choose Options

Anti-p53 antibody [BP53.12] (STJA0018375)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£1,230.50

Choose Options

Anti-p53 antibody [BP53.12] {FITC} (STJA0018374)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£1,981.50

Choose Options

Anti-p53 antibody [DO7] (STJ16100410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,009.50 - £1,055.50

Choose Options

Anti-p53 antibody [SRM] (STJ11107142)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Human colon carcinoma infiltrating fat stained with anti-p53 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of carcinoma cells.

Anti-p53 antibody [ZR153] (STJ180535)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £511.50

Choose Options

Immunofluorescence of paraffin embedded mouse skin using P63 at dilution of 1:500

Anti-p63 antibody [SM0486] (STJA0010486)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

Choose Options

Immunohistochemistry analysis of P63. Sample: Human cervical carcinoma, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200, RT, 1h.

Anti-p63 antibody [SM0508] (STJA0010508)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

Choose Options

Anti-p63 antibody [SM5049] (STJA0005049)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

Choose Options

Human skin stained with anti-p63 using peroxidase conjugate and DAB chromogen. Note nuclear staining of basal cells.

Anti-P63 antibody [ZM70] (STJ180314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£175.50 - £670.50

Choose Options

Immunohistochemistry analysis of p73 in paraffin-embedded human cervix cancer tissue using p73 Rabbit monoclonal antibody (STJ11103501) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-p73 antibody (1-100) [S1MR] (STJ11103501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of lysates from wild type (WT) and PAK2 knockout (KO) HeLa cells, using [KO Validated] PAK2 Rabbit polyclonal antibody (STJ11100066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAK2 antibody (1-380) (STJ11100066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of various cell lines, using PAK2 antibody (STJ29472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PAK2 antibody (120-240) (STJ29472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of 3T3 cells using PAK Gamma Polyclonal Antibody

Anti-PAK2 antibody (5-54 aa) (STJ94946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis using PAK Gamma monoclonal antibody against HeLa (1) , Jurkat (2) , A549 (3) , HEK293 (4) and K562 (5) cell lysate.

Anti-PAK2 antibody [3B5] (STJ98304)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£144.00 - £348.00

Choose Options

Immunohistochemistry analysis of paraffin embedded human pancreas using Pan-cadherin at dilution of 1:200

Anti-Pan-Cadherin antibody [SM4933] (STJA0004933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

Choose Options

Anti-Parkin antibody (C-Term) (STJ13100219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

Choose Options

Anti-Parkin antibody (C-Term) (STJ13100220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

Choose Options

Immunofluorescence analysis of U-251MG cells using ALIX/ PDCD6IP antibody (STJ24927) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PDCD6IP antibody (1-180) (STJ24927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PDCD6IP antibody (410-490 aa) (STJ194755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Anti-ALIX antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PDCD6IP antibody (840aa C-Term) (STJ140162)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

Choose Options

Anti-PELI1 antibody (STJA0007462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Thr445) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADD1-Thr445 antibody (411-460 aa) (STJ91115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot with Anti-Phospho-AKT1 Antibody (STJ502089) with PAKT peptide.1:250 dilution in buffer. From 10% SDS gel.

Anti-Phospho-AKT1 antibody (430-480) (STJ502089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£528.50

Choose Options

Anti-Phospho-AKT1 antibody (430-480) {Biotin} (STJ502090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£584.50

Choose Options

Anti-Phospho-AKT1 antibody (430-480) {FITC} (STJ502091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£584.50

Choose Options

Anti-Phospho-AKT1 antibody [M114] (STJA0005252)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-AKT1-S129 Rabbit pAb (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Anti-Phospho-AKT1-S212 antibody [SAA0279] (STJA0019075)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£378.50 - £1,632.50

Choose Options

Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [6F8] (STJ97726)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

Choose Options

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from C6 cells using Phospho-AKT1-S473 Rabbit pAb (STJ116369) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-AKT1-S473 antibody (STJ116369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Anti-Phospho-AKT1-S473 antibody [P8H9] (STJA0019074)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/AKT8 murine leukemia virus

£378.50 - £1,632.50

Choose Options

Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Anti-Phospho-AKT1-S473 antibody [SAA0300] (STJA0019165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

Choose Options

Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various cells using Phospho-Akt1 (S129) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser129 antibody (95-144 aa) (STJ90484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-AKT1-Ser129 antibody (STJ99594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options