Muscle Contraction | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using TNNT1 antibody (STJ112391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-TNNT1 antibody (1-262) (STJ112391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TNNT1 antibody (STJ191558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of mouse heart cells using Cardiac troponin T (TNNT2) Rabbit polyclonal antibody (STJ25910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TNNT2 antibody (1-120) (STJ25910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

€138.13 - €631.88

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Western blot analysis of AD293, 4T1 cells using Troponin T-C Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-TNNT2 antibody (131-180 aa) (STJ96727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of rat heart using Cardiac troponin T (TNNT2) Rabbit monoclonal antibody (STJ11102195) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TNNT2 antibody (199-298) [S5MR] (STJ11102195)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

€148.13 - €651.88

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Western blot analysis of various lysates using TNNT3 Rabbit polyclonal antibody (STJ117518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TNNT3 antibody (147-256) (STJ117518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TNNT3 antibody (STJ191557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells using Tropomyosin 1 antibody (STJ25940). Blue: DAPI for nuclear staining.

Anti-TPM1 antibody (1-245) (STJ25940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Western blot analysis of 1, mouse-lung 2, mouse-brain 3, mouse-spleen cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-TPM1 antibody (101-150 aa) (STJ99331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using Tropomyosin 1 Rabbit mAb (STJ11102362) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-TPM1 antibody (185-284) [S2MR] (STJ11102362)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Mouse/Rat

€148.13 - €651.88

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Western blot analysis of various cells using Tropomyosin Alpha Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-TPM1 antibody (40-89 aa) (STJ96111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunoprecipitation analysis of 300 Mu g extracts of Mouse skeletal muscle cells using 3 Mu g TPM2 antibody (STJ25941). Western blot was performed from the immunoprecipitate using TPM2 antibody (STJ25941) at a dilution of 1:1000.

Anti-TPM2 antibody (1-100) (STJ25941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-TPM2 antibody (STJA0008651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using TPM3 antibody (STJ115518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-TPM3 antibody (1-284) (STJ115518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€138.13 - €631.88

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Immunofluorescence analysis of HeLa cells using TPM3 antibody (STJ25942). Blue: DAPI for nuclear staining.

Anti-TPM3 antibody (1-285) (STJ25942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-TPM3 antibody (175-225) (STJA0006598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TPM4 antibody (127-177 aa) (STJ194771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of mouse skeletal muscle using TRIM72 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-TRIM72 antibody (STJ197325)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from Rat skeletal muscle, using Troponin I1/TnI1 Rabbit mAb (STJ11103673) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Troponin I1/TnI1 antibody (1-187) [S3MR] (STJ11103673)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€148.13 - €651.88

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Western blot analysis of extracts of various cell lines, using TRPC1 antibody (STJ114399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TRPC1 antibody (400-500) (STJ114399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Western blot analysis of extracts of various cell lines, using TRPC1 Rabbit monoclonal antibody (STJ11101983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TRPC1 antibody (400-500) [S3MR] (STJ11101983)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Anti-TRPC1 antibody (STJ191415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:200

Anti-TTN antibody (161-210 aa) (STJ98821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of paraffin-embedded mouse heart using TTN Rabbit polyclonal antibody (STJ119141) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TTN antibody (5347-5604) (STJ119141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

€138.13 - €631.88

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Anti-VCL antibody (100-200) [PT0372] (STJA0006223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of NIH/3T3 cells using Vinculin antibody (STJ116126) at dilution of 1:150. Blue: DAPI for nuclear staining.

Anti-VCL antibody (751-850) (STJ116126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-VCL antibody (786-835 aa) (STJ96247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Confocal imaging of HeLa cells using Vinculin Rabbit monoclonal antibody (STJ11101832, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-VCL antibody (800-900) [S2MR] (STJ11101832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Western blot analysis of Mouse brain, using Vinculin antibody (STJ11104807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-VCL antibody (800-900) [S4807RM] (STJ11104807)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €693.13

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Western blot analysis of various lysates using Vimentin Rabbit pAb (STJ26092) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-VIM antibody (1-200) (STJ26092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit polyclonal antibody (STJ113868) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (1-466) (STJ113868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-VIM antibody (367-466) [S7MR] (STJ11101727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Anti-VIM antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-VIM antibody (390aa C-Term) (STJ140133)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€169.38 - €493.13

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Human appendix tissue was stained with Anti-Vimentin (ABT019) Antibody

Anti-VIM antibody (400-466 aa) [ABT019] (STJ197249)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€225.00 - €326.25

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U2OS whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin (ABT281) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: U2OSPredicted band size: 54kDaObserved band size: 54kDa

Anti-VIM antibody (400-466 aa) [ABT281] (STJ197198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€225.00 - €326.25

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Immunocytochemistry analysis of Vimentin (green) in Hela using Vimentin antibody, and DAPI (blue).

Anti-VIM antibody (448-465 aa) [R02-8C4] (STJA0013017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat/Hamster

€330.63

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Immunofluorescence analysis of Hela cell. 1, Vimentin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-VIM antibody (56-105 aa) (STJ96245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot detection of Vimentin in Molt-4, K562, COS7, Jurkat, Hela and Vimentin negative cell (Daudi, Ramos, Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.

Anti-VIM antibody [4F8-A5-H5] (STJ99270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human

€186.25 - €450.00

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Anti-VIM/Vimentin antibody [SAA0112] (STJA0026948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€318.13 - €473.13

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Anti-VIM/Vimentin antibody [SAA0112] {APC} (STJA0029094)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€388.13 - €590.63

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Anti-VIM/Vimentin antibody [SAA0112] {FITC} (STJA0028128)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€318.13 - €473.13

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Anti-VIM/Vimentin antibody [SAA0112] {PerCP} (STJA0030060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€365.63 - €550.63

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Anti-VIM/Vimentin antibody [SAA0112] {PE} (STJA0031026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€388.13 - €590.63

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Anti-VIM/Vimentin antibody [VB6] (STJA0026947)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

€318.13 - €473.13

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Anti-VIM/Vimentin antibody [VB6] {APC} (STJA0029093)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

€388.13 - €590.63

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Anti-VIM/Vimentin antibody [VB6] {FITC} (STJA0028127)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

€318.13 - €473.13

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Anti-VIM/Vimentin antibody [VB6] {PerCP} (STJA0030059)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

€365.63 - €550.63

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Anti-VIM/Vimentin antibody [VB6] {PE} (STJA0031025)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

€388.13 - €590.63

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