Metabolism | Research Area | St John's Laboratory

Immunofluorescence analysis of L929 cells using NQO1 Rabbit polyclonal antibody (STJ24805) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NQO1 antibody (1-274) (STJ24805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using NQO1 Polyclonal Antibody diluted at 1：2000

Anti-NQO1 antibody (203-252) (STJ94551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NQO2 Rabbit monoclonal antibody (STJ11101959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NQO2 antibody (1-100) [S9MR] (STJ11101959)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using NQO2 Rabbit polyclonal antibody (STJ113336) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NQO2 antibody (1-231) (STJ113336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of NQO2 in paraffin-embedded Mouse kidney using NQO2 Rabbit polyclonal antibody (STJ113339) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NQO2 antibody (1-231) (STJ113339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of HeLa cells, using NQO2 antibody (STJ27393) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-NQO2 antibody (1-231) (STJ27393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NQO2 antibody (40-120 aa) (STJ191344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using Rev-Erb Alpha/NR1D1 Rabbit polyclonal antibody (STJ11100546) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NR1D1 antibody (280-360) (STJ11100546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-NR1D1 antibody (350-430 aa) (STJ95423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunocytochemistry analysis of NR1D1 (green) in Hela using NR1D1 antibody, and DAPI (blue)

Anti-NR1D1 antibody (591-605 aa) [R05-8B1] (STJA0011699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Western blot analysis of HeLa, using LXR beta/NER antibody (STJ11104867) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR1H2 antibody (1-100) (STJ11104867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using NR1H2 Rabbit polyclonal antibody (STJ11104520) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NR1H2 antibody (180-260) (STJ11104520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using NR1H2 Rabbit polyclonal antibody (STJ118723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-NR1H2 antibody (180-260) (STJ118723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NR1H2 antibody (STJ192129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Confocal imaging of C2C12 cells using LXR Alpha Rabbit monoclonal antibody (STJ11101976, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-NR1H3 antibody (1-100) [S6MR] (STJ11101976)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of HeLa cells using LXR Alpha Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NR1H3 antibody (151-200 aa) (STJ96508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

£48.00 - £252.00

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Western blot analysis of lysates from Mouse kidney, using LXR Alpha Rabbit polyclonal antibody (STJ24810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NR1H3 antibody (160-230) (STJ24810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Rat liver, using NR1H4 Rabbit polyclonal antibody (STJ114657) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NR1H4 antibody (1-270) (STJ114657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-NR1H4 antibody (1-50) (STJ501969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-NR1H4 antibody (1-50) {Biotin} (STJ501970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-NR1H4 antibody (1-50) {FITC} (STJ501971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Western blot analysis of various lysates using NR1H4 Rabbit pAb (STJ110618) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-NR1H4 antibody (207-476) (STJ110618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Huang, Jianbo, et al. "Effects of mulberry leaf on experimental hyperlipidemia rats induced by high‑fat diet. " Experimental and therapeutic medicine 16. 2 (2018) : 547-556.

Anti-NR1H4 antibody (STJ192265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NRF1 antibody (1-300) (STJ116123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 cells using NRF-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NRF1 antibody (221-270) (STJ94553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Bovine

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using NRF1 antibody (STJ27493) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-NRF1 antibody (240-449) (STJ27493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using NRF1 antibody (STJ11101843) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-NRF1 antibody (300-400) [S3MR] (STJ11101843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot detection of TCF11/NRF1 in Hela, K562, CEM and U2OS cell lysates using TCF11/NRF1 mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:72KDa.

Anti-NRF1 antibody [9E6-H8-B6] (STJ99173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] NSDHL Rabbit polyclonal antibody (STJ119221) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NSDHL antibody (1-240) (STJ119221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using NSDHL Rabbit monoclonal antibody (STJ11104204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NSDHL antibody (50-150) [S4204RM] (STJ11104204)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-NSDHL antibody (STJA0006988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Human cerebellum stained with anti-NSE antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of neuronal cells.

Anti-NSE antibody (416-433aa) [ZM24] (STJ180295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £515.50

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Anti-NSE antibody [MRQ-55] (STJ16100446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£722.50 - £1,214.50

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Anti-NSE antibody [SM5003] (STJA0005003)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-NT5C antibody (STJA0008522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using NT5C1A Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NT5C1A antibody (151-200) (STJ94563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using NT5C1B Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NT5C1B antibody (171-220 aa) (STJ91520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-NT5C2 antibody (STJ190738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-NT5C2 antibody (STJ500600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-NT5C2 antibody {Biotin} (STJ500601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-NT5C2 antibody {FITC} (STJ500602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NT5E antibody (27-574) (STJ24827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using CD73/NT5E rabbit polyclonal antibody (STJ11102804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NT5E antibody (32-264) (STJ11102804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of MCF7, L929, K562 cells using CD73 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NT5E antibody (431-480 aa) (STJ96619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) , cholangiocarcinorna (B) , lymph node (C) and esophagus (D) tissues with DAB staining using CD73 monoclonal antibody.

Anti-NT5E antibody [1D7] (STJ97935)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-NT5M antibody (STJA0009579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of NUBP1 in paraffin-embedded mouse kidney using NUBP1 Rabbit polyclonal antibody (STJ118831) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NUBP1 antibody (201-320) (STJ118831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NUBP2 antibody (STJA0008390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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