• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g HK1 antibody (STJ24019). Western blot was performed from the immunoprecipitate using HK1 antibody (STJ24019) at a dilution of 1:500.
  • Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] HK1 Rabbit polyclonal antibody (STJ24019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using [KO Validated] HK1 Rabbit polyclonal antibody (STJ24019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HK1 in paraffin-embedded mouse kidney using HK1 Rabbit polyclonal antibody (STJ24019) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HK1 in paraffin-embedded rat kidney using HK1 Rabbit polyclonal antibody (STJ24019) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit polyclonal antibody (STJ24019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] HK1 Rabbit polyclonal antibody (STJ24019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HK1 antibody (21-219) (STJ24019)

SKU:
STJ24019

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HK1 (21-219) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HK1
Gene ID: 3098
Uniprot ID: HXK1_HUMAN
Immunogen Region: 21-219
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_000179.2).
Immunogen Sequence: KIDKYLYAMRLSDETLIDIM TRFRKEMKNGLSRDFNPTAT VKMLPTFVRSIPDGSEKGDF IALDLGGSSFRILRVQVNHE KNQNVHMESEVYDTPENIVH GSGSQLFDHVAECLGDFMEK RKIKDKKLPVGFTFSFPCQQ SKIDEAILITWTKRFKASGV EGADVVKLLNKAIKKRGDYD ANIVAVVNDTVGTMMTCGY
Tissue Specificity Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.
Function Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
Protein Name Hexokinase-1
Brain Form Hexokinase
Hexokinase Type I
Hk I
Hexokinase-A
Database Links Reactome: R-HSA-5619056
Reactome: R-HSA-70171
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
The Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)
Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-Glucosamine
Leading To Relocation To The Cytosol
Alternative Antibody Names Anti-Hexokinase-1 antibody
Anti-Brain Form Hexokinase antibody
Anti-Hexokinase Type I antibody
Anti-Hk I antibody
Anti-Hexokinase-A antibody
Anti-HK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance