Metabolism | Research Area | St John's Laboratory

Western blot analysis of A-431, using ELOVL4 antibody (STJ23530) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ELOVL4 antibody (1-100) (STJ23530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry of human tissue with Anti-ELOVL4 primary antibody (STJ500876) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ELOVL4 antibody (107-121) (STJ500876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-ELOVL4 antibody (107-121) {Biotin} (STJ500874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-ELOVL4 antibody (107-121) {FITC} (STJ500875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Immunohistochemistry with Anti-ELOVL4 Antibody (STJ500873) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-ELOVL4 antibody (2-22) (STJ500873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Cat/Human/Monkey/Rat

£400.50

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Anti-ELOVL4 antibody (2-22) {Biotin} (STJ500880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Cat/Human/Monkey/Rat

£497.50

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Anti-ELOVL4 antibody (2-22) {FITC} (STJ500881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Cat/Human/Monkey/Rat

£497.50

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Anti-ELOVL4 antibody (294-301) (STJ500879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Pig/Rat

£400.50

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Anti-ELOVL4 antibody (294-301) {Biotin} (STJ500877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Pig/Rat

£497.50

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Anti-ELOVL4 antibody (294-301) {FITC} (STJ500878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Pig/Rat

£497.50

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Western blot analysis of various cells using ELOVL4 Polyclonal Antibody diluted at 1：1000

Anti-ELOVL4 antibody (41-90 aa) (STJ92903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HepG2 cells, using ELOVL5 Rabbit polyclonal antibody (STJ118994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ELOVL5 antibody (247-326) (STJ118994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ELOVL5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ELOVL5 antibody (250-299 aa) (STJ92904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ELOVL5 antibody [R07-7B3] (STJA0033772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various cells using ELOVL6 Polyclonal Antibody

Anti-ELOVL6 antibody (21-70 aa) (STJ92905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Mouse kidney using ELOVL6 Rabbit polyclonal antibody (STJ11104179) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 30 s.

Anti-ELOVL6 antibody (50-150) (STJ11104179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EMAP II antibody [R01-5A9] (STJA0034275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£140.50 - £308.50

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Anti-EMAP-II/AIMP1 antibody (1-336aa) [AT6D10] (STJA0041770)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of extracts of various cell lines, using ENO1 Rabbit monoclonal antibody (STJ11101333) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ENO1 antibody (1-100) [S3MR] (STJ11101333)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using ENO1 rabbit polyclonal antibody (STJ11101183) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ENO1 antibody (1-434) (STJ11101183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g ENO1 Rabbit polyclonal antibody (STJ23538). Western blot was performed from the immunoprecipitate using ENO1 antibody (STJ23538) at a dilution of 1:1000.

Anti-ENO1 antibody (1-434) (STJ23538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ENO1 antibody (120-170 aa) (STJ192921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ENO1 antibody [3F7-B12-D10] (STJA0032498)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£133.50 - £281.50

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Anti-ENO1 antibody [R01-4C8] (STJA0033953)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-ENO1/2/3 antibody [R09-9H5] (STJA0034279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-ENO1/Alpha-enolase antibody (Met1-Lys434) [1A067] (STJA0021905)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£211.50 - £290.50

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Western blot analysis of various lysates, using ENO2 antibody (STJ23539) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ENO2 antibody (1-127) (STJ23539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using ENO2 antibody (STJ114221) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ENO2 antibody (195-434) (STJ114221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-NSE antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 47 kDa

Anti-ENO2 antibody (300-434 aa) [ABT-NSE] (STJ197038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Human colon tissue was stained with Anti-NSE (PT2281) Antibody

Anti-ENO2 antibody (300-434 aa) [PT2281] (STJ197039)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Enolase Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-ENO2 antibody (371-420 aa) (STJ92926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using ENO2 antibody (STJ11104813) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ENO2 antibody (47-100) [S4813RM] (STJ11104813)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using ENO2 antibody (STJ11101628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ENO2 antibody (47-100) [S8MR] (STJ11101628)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of Hela cell. 1, Cdk2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ENO2 antibody [13E2] (STJ96968)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ENO2 antibody [BPM6150] (STJA0033398)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£214.50 - £308.50

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Western blot analysis of various lysates using ENO3 Rabbit mAb (STJ11107357) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-ENO3 antibody (1-100 aa) [SRM5444] (STJ11107357)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of PC-12 cells using ENO3 antibody (STJ118799) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ENO3 antibody (1-190) (STJ118799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using ENO3 antibody (STJ23540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ENO3 antibody (1-190) (STJ23540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ENO3 antibody (STJ190609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ENO3 antibody [R03-9R-6] (STJA0033093)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£214.50 - £308.50

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Anti-ENO3 antibody [R07-5B2] (STJA0034278)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-ENO3 antibody [SRM13456] (STJ11108186)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-ENO4 antibody (STJA0004306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Anti-ENOA antibody [PT1286R] (STJA0040042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Enolase 1/2 antibody (1-434aa) [AT1G7] (STJA0041798)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-Enolase 2/ENO2 antibody (1-434aa) [AT17D10] (STJA0041667)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-Enolase antibody (Internal Region) (STJ13100198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse/Marmoset

£322.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ENOPH1 antibody (112-162 aa) (STJ194120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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