Metabolism Of Proteins | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CFTR-Ser737 antibody (STJ193221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CLSPN-Ser30 antibody (STJA0009982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells treated with UV 15', using RFWD2 (Phospho-Ser387) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-COP1-Ser387 antibody (353-402 aa) (STJ90637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CSF2RB-Tyr593 antibody (559-608 aa) (STJ90799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293F cells using Phospho-Casein Kinase 2 alpha (CSNK2A1) -T360/S362 Rabbit pAb (STJ22081) at 1:400 dilution. 293F cells were treated by ERK2 (100ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CSNK2A1-T360/S362 antibody (STJ22081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of HeLa cells using Phospho-Casein Kinase II Alpha (Y255) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-CSNK2A1-Tyr255 antibody (221-270 aa) (STJ90551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HuvEc cells using Phospho-Casein Kinase II Beta (S209) Polyclonal Antibody

Anti-Phospho-CSNK2B-Ser209 antibody (166-215 aa) (STJ90892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of JK cells using Phospho-CtBP1 (S422) Polyclonal Antibody

Anti-Phospho-CTBP1-Ser422 antibody (388-437 aa) (STJ90975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates, using Phospho-Beta-Catenin-S29/33/37/T41 Rabbit pAb (STJ11101114) at 1:400 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CTNNB1-S29/33/37/T41 antibody (STJ11101114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-Beta-Catenin-S33/S37 Rabbit mAb (STJ11102571) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CTNNB1-S33/S37 antibody [S1MR] (STJ11102571)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Beta-Catenin-S33/S37/T41 Rabbit pAb (STJ11100967) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-CTNNB1-S33/S37/T41 antibody (STJ11100967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Beta-Catenin-S45 Rabbit pAb (STJ29346) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-S45 antibody (STJ29346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-Beta-Catenin-S552 Rabbit pAb (STJ29345) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-CTNNB1-S552 antibody (STJ29345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Beta-Catenin-S552 antibody (STJ11105815) at 1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CTNNB1-S552 antibody [S5815RM] (STJ11105815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-Beta-Catenin-S675 Rabbit pAb (STJ117893) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-CTNNB1-S675 antibody (STJ117893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Ser33 antibody (17-66 aa) (STJ90206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-CTNNB1-Ser37 antibody (3-52 aa) (STJ90207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from NIH-3T3 cells, using Phospho-Catenin-Beta (S552) Polyclonal Antibody.

Anti-Phospho-CTNNB1-Ser552 antibody (STJ91364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CTNNB1-Ser675 antibody (STJ196478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SW626 cells using Phospho-Beta-Catenin-T41/S45 Rabbit pAb (STJ22085). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-T41/S45 antibody (STJ22085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-Catenin-Beta (T41/S45) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CTNNB1-Thr41/S45 antibody (11-60 aa) (STJ90208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-CTNNB1-Tyr-142 antibody (STJA0005304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CTNNB1-Tyr-333 antibody (STJA0005305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-CTNNB1-Tyr-489 antibody (STJA0005306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CTNNB1-Tyr-654 antibody (STJA0005307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-CTNNB1-Tyr-86 antibody (STJA0005303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Tyr489 antibody (455-504 aa) (STJ90928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-Catenin-Beta (Y654) Polyclonal Antibody

Anti-Phospho-CTNNB1-Tyr654 antibody (620-669 aa) (STJ90209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293 cells, using Phospho-Beta-Catenin-Y333 Rabbit pAb (STJ22086). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-Y333 antibody (STJ22086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Western Blot analysis of 1 A431 treated with LPS, 2 A431, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CYLD-Ser418 antibody (STJ196281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-DAG1-Tyr-892 antibody [M117] (STJA0005378)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of 293 cells using Phospho-Daxx (S668) Polyclonal Antibody

Anti-Phospho-DAXX-Ser668 antibody (634-683 aa) (STJ90785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-DDX5-Tyr593 antibody (565-614 aa) (STJ91304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of MCF-7 cells using Phospho-DNMT1-S714 Rabbit pAb (STJ22088). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-DNMT1-pS714 antibody (STJ22088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-DNMT1-pY399 antibody (STJ22089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~50 kDa eEF1A2 protein phosphorylated at Ser358 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 min).

Anti-Phospho-EEF1A2-Ser358 antibody (STJA0003624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Chicken/Non-Human Primates/Rat/Sheep/Xenopus

£454.50

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Western blot analysis of lysates from 293T cells, using Phospho-eEF2-T56 Rabbit pAb (STJ11100973) at 1:3000 dilution. 293T cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-EEF2-T56 antibody (STJ11100973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-EEF2-Thr56 antibody (31-80 aa) (STJ91030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-EEF2-Thr56 antibody (STJ99592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (A4869) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-EIF2AK2-T446 antibody [S1MR] (STJ11102631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKR (Phospho-Thr258) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EIF2AK2-Thr258 antibody (226-275 aa) (STJ90956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells treated with IFN 2500U/ml 30', using PKR (Phospho-Thr446) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EIF2AK2-Thr446 antibody (413-462 aa) (STJ90392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKR (Phospho-Thr451) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EIF2AK2-Thr451 antibody (418-467 aa) (STJ90393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-EIF2B5-Ser540 antibody (STJA0009946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-eIF2 Alpha-S49 Rabbit pAb (STJ22112). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-EIF2S1-S49 antibody (STJ22112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-eIF2 Alpha-S51 Rabbit pAb (STJ23509). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-EIF2S1-S51 antibody (STJ116376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-eIF2 Alpha-S51 Rabbit mAb (STJ11102562) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-EIF2S1-S51 antibody [S2MR] (STJ11102562)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-eIF2α (Phospho-Ser51) (PTR1316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 40kDaObserved band size: 40, 90kDa

Anti-Phospho-EIF2S1-Ser51 antibody (1-100) [PTR1316] (STJA0006271)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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