Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-H2AX-Ser139 antibody (STJA0009882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£144.00 - £348.00

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Anti-Phospho-H2AX-Thr120 antibody (STJ197460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-H2AX-Tyr142 antibody (STJ197459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-H2BC3-Ser-36 antibody (STJA0005438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-H2BC9-Ser14 antibody (STJ194711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-H3-3A/H3-3B-Ser31 antibody (16-65 aa) (STJ91330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-H4C1-Tyr-72 antibody (STJA0005439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using HDAC1 (Phospho-Ser421) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC1-Ser421 antibody (387-436 aa) (STJ90497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells, using Phospho-HDAC2-S394 Rabbit pAb (STJ11105771) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-HDAC2-S394 antibody (STJ11105771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-HDAC2 (S394) Polyclonal Antibody

Anti-Phospho-HDAC2-Ser394 antibody (360-409 aa) (STJ90442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot of mouse heart lysate showing specific immunolabeling of the ~55 kDa HDAC2 protein phosphorylated at Ser394 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-HDAC2-Ser394 antibody (STJA0003670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Chicken/Guinea Pig/Human/Non-Human Primates/Sheep

£454.50

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Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC3-Ser424 antibody (379-428 aa) (STJ91081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293 cells using Phospho-HDAC4-S632 Rabbit pAb (STJ22165). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-HDAC4-S632 antibody (STJ22165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of Jurkat cells, using Phospho-HDAC4-S632 antibody (STJ11105833) at1:2000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-HDAC4-S632 antibody [S5833RM] (STJ11105833)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various cells using Phospho-HDAC4 (S632) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-HDAC4-Ser632 antibody (598-647 aa) (STJ90285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using HDAC7A (Phospho-Ser155) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC7-Ser155 antibody (121-170 aa) (STJ90655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-HDAC7-Ser486 antibody (STJA0010043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Hrs (Y216) Polyclonal Antibody

Anti-Phospho-HGS-Tyr216 antibody (STJ90292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HGS-Tyr334 antibody (301-350 aa) (STJ91095)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (STJ27513) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-Histone H2A-S139 antibody (STJ27513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Histone H2A-S2 antibody (STJ110882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunohistochemistry of baboon spleen with Anti-Phospho-Histone H3 primary antibody (S10) (STJ502483) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-Phospho-Histone H3 antibody (1-50) (STJ502483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£528.50

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Anti-Phospho-Histone H3 antibody (1-50) {Biotin} (STJ502484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£584.50

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Anti-Phospho-Histone H3 antibody (1-50) {FITC} (STJ502485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£584.50

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Anti-Phospho-Histone H3-S32 antibody (STJ29330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Histone H3 (Phospho-Thr3) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Histone H3.1-Thr3 antibody (1-50 aa) (STJ90291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (STJ11101036) at 1:1000 dilution or Histone H4 Rabbit pAb (STJ29382). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-Histone H4-S1 antibody (STJ11101036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Histone H4-Ser1 antibody (STJ197451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells, using Histone H4 (Phospho-Ser47) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Histone H4-Ser47 antibody (13-62 aa) (STJ91343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Histone H4-Thr80 antibody (STJ197450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-hnRNP C1/2 (S260) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-HNRNPC-Ser260 antibody (231-280 aa) (STJ90910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-HNRNPK-Ser216 antibody (STJ90631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody

Anti-Phospho-HNRNPK-Ser284 antibody (250-299 aa) (STJ90527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-HTRA2-Ser142 antibody (STJ91278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-HTRA2-Ser212 antibody (181-230 aa) (STJ91257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-IGFBP3-Ser183 antibody (151-200 aa) (STJ91161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-IKBKE-Ser172 antibody (STJ196329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-IKK Gamma-S376 Rabbit pAb (STJ11101145) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-IKBKG-S376 antibody (STJ11101145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-IKK Gamma (S31) Polyclonal Antibody

Anti-Phospho-IKBKG-Ser31 antibody (16-65 aa) (STJ90798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using IKK-gamma (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IKBKG-Ser376 antibody (342-391 aa) (STJ91203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells treated with Anisomycin 0.5uM 5h, using IKK-gamma (Phospho-Ser85) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-IKBKG-Ser85 antibody (51-100 aa) (STJ90543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-INCENP-Thr59 antibody (STJA0009973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-KAP1/TRIM28-S824 antibody (STJ11107705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-TAK1-S412 Rabbit pAb (STJ22254) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 120s.

Anti-Phospho-MAP3K7-S412 antibody (STJ22254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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NLRC5 Deficiency Reduces LPS-Induced Microglial Activation via Inhibition of NF-κB Signaling and Ameliorates Mice’s Depressive-like Behavior. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Jianqiong Zhang WB mouse 1:500 BV2 cell

Anti-Phospho-MAP3K7-Ser412 antibody (STJ196446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T using p-Tak1 (S439) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP3K7-Ser439 antibody (411-460 aa) (STJ90947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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