• Immunohistochemistry analysis of LAMC1 in paraffin-embedded human uterine cancer using LAMC1 Rabbit polyclonal antibody (STJ118460) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LAMC1 in paraffin-embedded human uterus using LAMC1 Rabbit polyclonal antibody (STJ118460) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LAMC1 in paraffin-embedded human breast cancer using LAMC1 Rabbit polyclonal antibody (STJ118460) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from LO2 cells, using LAMC1 Rabbit polyclonal antibody (STJ118460) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using LAMC1 Rabbit polyclonal antibody (STJ118460) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LAMC1 antibody (1030-1608) (STJ118460)

SKU:
STJ118460

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LAMC1 (1030-1608) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAMC1
Gene ID: 3915
Uniprot ID: LAMC1_HUMAN
Immunogen Region: 1030-1608
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1608 of human LAMC1 (NP_002284.3).
Immunogen Sequence: ECPACYRLVKDKVADHRVKL QELESLIANLGTGDEMVTDQ AFEDRLKEAEREVMDLLREA QDVKDVDQNLMDRLQRVNNT LSSQISRLQNIRNTIEETGN LAEQARAHVENTERLIEIAS RELEKAKVAAANVSVTQPES TGDPNNMTLLAEEARKLAER HKQEADDIVRVAKTANDTST EAYNLLLRTLAGENQTAFEI EELNRKYEQAKNISQDLEKQ AARVHEEAKRAGDKAVEIY
Tissue Specificity Found in the basement membranes (major component).
Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Protein Name Laminin Subunit Gamma-1
Laminin B2 Chain
Laminin-1 Subunit Gamma
Laminin-10 Subunit Gamma
Laminin-11 Subunit Gamma
Laminin-2 Subunit Gamma
Laminin-3 Subunit Gamma
Laminin-4 Subunit Gamma
Laminin-6 Subunit Gamma
Laminin-7 Subunit Gamma
Laminin-8 Subunit Gamma
Laminin-9 Subunit Gamma
S-Laminin Subunit Gamma
S-Lam Gamma
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-3000157
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-373760
Reactome: R-HSA-381426
Reactome: R-HSA-8874081
Reactome: R-HSA-8957275
Reactome: R-HSA-9619665
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative Antibody Names Anti-Laminin Subunit Gamma-1 antibody
Anti-Laminin B2 Chain antibody
Anti-Laminin-1 Subunit Gamma antibody
Anti-Laminin-10 Subunit Gamma antibody
Anti-Laminin-11 Subunit Gamma antibody
Anti-Laminin-2 Subunit Gamma antibody
Anti-Laminin-3 Subunit Gamma antibody
Anti-Laminin-4 Subunit Gamma antibody
Anti-Laminin-6 Subunit Gamma antibody
Anti-Laminin-7 Subunit Gamma antibody
Anti-Laminin-8 Subunit Gamma antibody
Anti-Laminin-9 Subunit Gamma antibody
Anti-S-Laminin Subunit Gamma antibody
Anti-S-Lam Gamma antibody
Anti-LAMC1 antibody
Anti-LAMB2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance