Anti-KNG1 antibody (545-644) [S4MR] (STJ11101374)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Kininogen 1 (545-644) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KNG1 |
| Gene ID | 3827 |
| Uniprot ID | KNG1_HUMAN |
| Immunogen Region | 545-644 |
| Immunogen Sequence | PTPIPSLAKPGVTVTFSDFQ DSDLIATMMPPISPAPIQSD DDWIPDIQIDPNGLSFNPIS DFPDTTSPKCPGRPWKSVSE INPTTQMKESYYFDLTDGLS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Kininogen 1 (KNG1) (NP_001095886.1). |
Additional Info
| Tissue Specificity | Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors. |
| Post Translational Modifications | Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus. Bradykinin is released from kininogen by plasma kallikrein. Hydroxylation of Pro-383 occurs prior to the release of bradykinin. Phosphorylated by FAM20C in the extracellular medium. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. (Microbial infection) Bradykinin is generated upon proteolytic cleavage by S.pyogenes SpeB to produce hypotension during septic shock. |
| Function | Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action). |
| Protein Name | Kininogen-1Alpha-2-Thiol Proteinase InhibitorFitzgerald FactorHigh Molecular Weight KininogenHmwkWilliams-Fitzgerald-Flaujeac Factor Cleaved Into - Kininogen-1 Heavy Chain - T-KininIle-Ser-Bradykinin - BradykininKallidin I - Lysyl-BradykininKallidin Ii - Kininogen-1 Light Chain - Low Molecular Weight Growth-Promoting Factor |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140837Reactome: R-HSA-375276Reactome: R-HSA-381426Reactome: R-HSA-416476Reactome: R-HSA-418594Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Kininogen-1 antibodyAnti-Alpha-2-Thiol Proteinase Inhibitor antibodyAnti-Fitzgerald Factor antibodyAnti-High Molecular Weight Kininogen antibodyAnti-Hmwk antibodyAnti-Williams-Fitzgerald-Flaujeac Factor Cleaved Into - Kininogen-1 Heavy Chain - T-Kinin antibodyAnti-Ile-Ser-Bradykinin - Bradykinin antibodyAnti-Kallidin I - Lysyl-Bradykinin antibodyAnti-Kallidin Ii - Kininogen-1 Light Chain - Low Molecular Weight Growth-Promoting Factor antibodyAnti-KNG1 antibodyAnti-BDK antibodyAnti-KNG antibody |
Information sourced from Uniprot.org