• Western blot analysis of various lysates using Kininogen 1 (KNG1) (KNG1) Rabbit monoclonal antibody (STJ11101374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using Kininogen 1 (KNG1) (KNG1) Rabbit monoclonal antibody (STJ11101374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KNG1 antibody (545-644) [S4MR] (STJ11101374)

SKU:
STJ11101374

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Kininogen 1 (545-644) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KNG1
Gene ID: 3827
Uniprot ID: KNG1_HUMAN
Immunogen Region: 545-644
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Kininogen 1 (KNG1) (NP_001095886.1).
Immunogen Sequence: PTPIPSLAKPGVTVTFSDFQ DSDLIATMMPPISPAPIQSD DDWIPDIQIDPNGLSFNPIS DFPDTTSPKCPGRPWKSVSE INPTTQMKESYYFDLTDGLS
Tissue Specificity Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
Post Translational Modifications Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus. Bradykinin is released from kininogen by plasma kallikrein. Hydroxylation of Pro-383 occurs prior to the release of bradykinin. Phosphorylated by FAM20C in the extracellular medium. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. (Microbial infection) Bradykinin is generated upon proteolytic cleavage by S.pyogenes SpeB to produce hypotension during septic shock.
Function Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
Protein Name Kininogen-1
Alpha-2-Thiol Proteinase Inhibitor
Fitzgerald Factor
High Molecular Weight Kininogen
Hmwk
Williams-Fitzgerald-Flaujeac Factor Cleaved Into - Kininogen-1 Heavy Chain - T-Kinin
Ile-Ser-Bradykinin - Bradykinin
Kallidin I - Lysyl-Bradykinin
Kallidin Ii - Kininogen-1 Light Chain - Low Molecular Weight Growth-Promoting Factor
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140837
Reactome: R-HSA-375276
Reactome: R-HSA-381426
Reactome: R-HSA-416476
Reactome: R-HSA-418594
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Kininogen-1 antibody
Anti-Alpha-2-Thiol Proteinase Inhibitor antibody
Anti-Fitzgerald Factor antibody
Anti-High Molecular Weight Kininogen antibody
Anti-Hmwk antibody
Anti-Williams-Fitzgerald-Flaujeac Factor Cleaved Into - Kininogen-1 Heavy Chain - T-Kinin antibody
Anti-Ile-Ser-Bradykinin - Bradykinin antibody
Anti-Kallidin I - Lysyl-Bradykinin antibody
Anti-Kallidin Ii - Kininogen-1 Light Chain - Low Molecular Weight Growth-Promoting Factor antibody
Anti-KNG1 antibody
Anti-BDK antibody
Anti-KNG antibody

Information sourced from Uniprot.org

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