Metabolism Of Proteins | Research Area | St John's Laboratory

Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BACE1-Ser498 antibody (STJA0009813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-BAP1-Ser592 antibody (STJ196250)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western Blot analysis of K-562 Jurkat using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-BCL10-Ser138 antibody (STJA0009952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (STJ11103769) at 1:500 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Beclin 1-S15 antibody (STJ11103769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from C6 cells using Phospho-Beclin 1-Ser90 Rabbit pAb (STJ11103771) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Beclin 1-S90 antibody (STJ11103771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-Beclin 1-S90 antibody [SRM5546] (STJ11107736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Western blot analysis of lysates from HeLa cells, using Phospho-Beclin 1-Ser93 Rabbit pAb (STJ11103786) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Beclin 1-S93 antibody (STJ11103786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Anti-Phospho-BECN1-Ser15 antibody (STJ196252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot beclin-1 phospho-Ser234 immunolabeling in wild type vs mutant HeLa lysate STJA0003586

Anti-Phospho-BECN1-Ser234 antibody (STJA0003586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Non-Human Primates/Sheep

€586.20

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Anti-Phospho-BECN1-Ser93 antibody (STJ196253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western Blot analysis of mouse spleen, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BECN1-Ser93/96 antibody (STJA0010075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Phospho-beta Arrestin 1 Ser412 antibody western blot A549 MCF-7 U251 HL-60 STJA0035053

Anti-Phospho-beta Arrestin 1-Ser412 antibody [R05-6F7] (STJA0035053)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

€160.20 - €337.80

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Phospho-beta Catenin antibody western blot 293T lysates STJA0036213

Anti-Phospho-beta Catenin-Ser33/Ser37 antibody [R03-5O3] (STJA0036213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€160.20 - €337.80

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Anti-Phospho-Beta-Catenin-Tyr-142 antibody (STJA0041137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-333 antibody (STJA0041158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-489 antibody (STJA0041156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-654 antibody (STJA0041163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-86 antibody (STJA0041141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Beta-Dystroglycan-Tyr-892 antibody [M117] (STJA0041171)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Beta-Tubulin-Ser-172 antibody (STJA0041423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€651.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using Survivin (Phospho-Thr117) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BIRC5-Thr117 antibody (86-135 aa) (STJ91292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HT29 cells treated with EGF 100 μg/ml for 30 min, using Survivin (Phospho-Thr34) STJ90479

Anti-Phospho-BIRC5-Thr34 antibody (11-60 aa) (STJ90479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BLM-Thr99 antibody (65-114 aa) (STJ91066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from HT-29 cells, using Phospho-BRCA1-S1423 Rabbit pAb (STJ22043) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-BRCA1-S1423 antibody (STJ22043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-BRCA1-S988 Rabbit pAb (STJ22045) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-BRCA1-S988 antibody (STJ22045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human

€151.80 - €742.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1423) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1423 antibody (1391-1440 aa) (STJ90198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

€58.80 - €313.20

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Western blot analysis of lysates from 293 cells treated with epo 20U/ml 15', using BRCA1 (Phospho-Ser1457) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1457 antibody (1423-1472 aa) (STJ90622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western Blot analysis of mouse brain rat brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BRCA1-Ser1497 antibody (STJA0010038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1524) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1524 antibody (1491-1540 aa) (STJ90199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-BRCA1-Ser988 antibody (STJA0009869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Calnexin (S583) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CANX-Ser583 antibody (543-592 aa) (STJ90201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HP1 alpha (Phospho-Ser92) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CBX5-Ser92 antibody (58-107 aa) (STJ91233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western Blot analysis of HeLa mouse heart using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-CCNE1-Ser399 antibody (STJA0010041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using Phospho-Cyclin E1-T395 Rabbit pAb (STJ22052). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CCNE1-T395 antibody (STJ22052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cyclin E1-T77 Rabbit mAb (STJ11102599) at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CCNE1-T77 antibody [S9MR] (STJ11102599)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€172.20 - €742.20

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Western blot analysis of various cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CCNE1-Thr395 antibody (361-410 aa) (STJ90241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-CCNE1-Thr62 antibody (STJ196280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). STJ91326

Anti-Phospho-CCNE1-Thr77 antibody (43-92 aa) (STJ91326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of Cyclin E2 (Phospho-Thr392) Antibody. The lane on the right is blocked with the Cyclin E2 (Phospho-Thr392) peptide.

Anti-Phospho-CCNE2-Thr392 antibody (355-404 aa) (STJ91333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Anti-Phospho-CDC20-Ser51 antibody (STJ196268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of 293 cells using Phospho-Cdc25A (S124) Polyclonal Antibody

Anti-Phospho-CDC25A-Ser124 antibody (90-139 aa) (STJ90511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CDC25A-Ser178 antibody (144-193 aa) (STJ90929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of Jurkat cells using Phospho-Cdc25A (S75) Polyclonal Antibody

Anti-Phospho-CDC25A-Ser75 antibody (43-92 aa) (STJ90216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Cdc25A (S75) Polyclonal Antibody

Anti-Phospho-CDC25A-Ser75 antibody (STJ90215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-CDC25A-Thr507 antibody (STJ90564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-CDH2-Tyr-820 antibody (STJA0005287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€438.60

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Anti-Phospho-CDH2-Tyr-860 antibody (STJA0005290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€438.60

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T14 Rabbit pAb (STJ22058) at 1:500 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CDK1-T14 antibody (STJ22058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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