Anti-Phospho-CCNE1-Thr395 antibody (361-410 aa) (STJ90241)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Cyclin E1 around the phosphorylation site of Thr395 at the amino acid range 361-410
General Information
| Short Description | Rabbit polyclonal anti-Phospho-G1/S-specific cyclin-E1-Thr395 (361-410 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCNE1 |
| Gene ID | 898 |
| Uniprot ID | CCNE1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Cyclin E1 around the phosphorylation site of Thr395 at the amino acid range 361-410 |
| Immunogen Region | 361-410 aa |
| Specificity | Phospho-Cyclin E1 (T395) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T395. |
Additional Info
| Post Translational Modifications | Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation. |
| Function | Essential for the control of the cell cycle at the G1/S (start) transition. |
| Protein Name | G1/S-Specific Cyclin-E1 |
| Database Links | Reactome: R-HSA-1538133Reactome: R-HSA-187577Reactome: R-HSA-2559586Reactome: R-HSA-390471Reactome: R-HSA-6804116Reactome: R-HSA-69017Reactome: R-HSA-69200Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-8849470Reactome: R-HSA-9661069Reactome: R-HSA-9706019 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-E1 antibodyAnti-CCNE1 antibodyAnti-CCNE antibody |
Information sourced from Uniprot.org