Immune System | Research Area | St John's Laboratory

Anti-AGO1 antibody (STJA0007760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using Argonaute-2 antibody (STJ27819) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AGO2 antibody (1-100) (STJ27819)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Argonaute-2 antibody (STJ11101778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AGO2 antibody (350-450) [S8MR] (STJ11101778)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Argonaute 2 antibody immunocytochemistry HCT116 cells STJA0010979

Anti-AGO2 antibody (381-396 aa) [R04-5H0] (STJA0010979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of L929 cells using Argonaute-2 Polyclonal Antibody (STJ29944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AGO2 antibody (47-115) (STJ29944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AGO2 antibody (STJA0009725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-AGO2 antibody [PT0848R] (STJA0039160)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-AGO3 antibody (STJ190813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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AGO3 antibody western blot EIF2C3 human brain STJA0036116

Anti-AGO3 antibody [R09-2A1] (STJA0036116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-AGO4 antibody (STJA0004797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Immunofluorescence analysis of HeLa cells using AGPAT2 antibody (STJ28601). Blue: DAPI for nuclear staining.

Anti-AGPAT2 antibody (199-278) (STJ28601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using AHCYL1 antibody (STJ110084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AHCYL1 antibody (31-260) (STJ110084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AHCYL1 antibody (STJA0006806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Fetuin A/Fetuin A/AHSG antibody (STJ22552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AHSG antibody (19-300) (STJ22552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-AHSG antibody (STJA0010199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-AHSG antibody (STJA0010206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-AHSG antibody (STJA0010225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of A-431 cells using AIM2 Rabbit polyclonal antibody (STJ22558) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AIM2 antibody (130-337) (STJ22558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot with Anti-AIM2 Antibody (STJ500057) of mouse cortex at 1:500 dilution in buffer.

Anti-AIM2 antibody (240-340) (STJ500057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£400.50

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Anti-AIM2 antibody (240-340) {Biotin} (STJ500058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£497.50

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Western blot with Anti-AIM2 Antibody (FITC) (STJ500059) of cerebral cortex at 1:500 dilution in buffer.

Anti-AIM2 antibody (240-340) {FITC} (STJ500059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£497.50

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Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-AIM2 antibody (51-100 aa) (STJ91516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-AIM2 antibody (N-Term) (STJA0005250)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot AIM2 detection in Jurkat cells and mouse macrophages using antibody STJA0041069

Anti-AIM2 antibody (N-Term) (STJA0041069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-AIM2 antibody [PT1057R] (STJA0039969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts of various cell lines, using AIP antibody (STJ114420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-AIP antibody (1-330) (STJ114420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AIP antibody (251-301 aa) (STJ194443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot WB analysis A431 cells using anti-Akt antibody protein detection STJA0041047

Anti-Akt antibody (N-Term) [M101] (STJA0041047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-AKT antibody (STJ13100006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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AKT antibody immunohistochemistry human lung carcinoma STJA0032920

Anti-AKT antibody [2A1-7G2-3F4] (STJA0032920)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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AKT antibody western blot 3T3 rat heart STJA0033993

Anti-AKT antibody [R08-8F1] (STJA0033993)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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ICC IF of AKT1 3 in A549 cells stained with Human Akt1 antibody STJA0041853

Anti-Akt1 antibody (1-480aa) [AT6D5] (STJA0041853)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Akt1 antibody (251-351 aa) [SRM5087] (STJ11106868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunoprecipitation analysis of 300 Mu g extracts from MCF7 cells using 3 Mu g AKT1 Rabbit monoclonal antibody (STJ11101453). Western blot was performed from the immunoprecipitate using AKT1 Rabbit monoclonal antibody (STJ11101453) at a dilution of 1:1000.

Anti-AKT1 antibody (381-480) [S3MR] (STJ11101453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts from wild type (WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (STJ11103457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AKT1 antibody (381-480) [S7MR] (STJ11103457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry with Anti-AKT1 Antibody (STJ500060) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-AKT1 antibody (C-Term) (STJ500060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Western blot analysis using Akt1 monoclonal antibody against C2C12, K562 cell lysate.

Anti-AKT1 antibody (C-Term) (STJ98461)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

£149.00 - £360.00

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Anti-AKT1 antibody (C-Term) {Biotin} (STJ500061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-AKT1 antibody (C-Term) {FITC} (STJ500062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-AKT1 antibody (N-Term) [M101] (STJA0005251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000) , with super ECL.Predicted band size:60KDa.Observed band size:60KDa.

Anti-AKT1 antibody [2D9-D2-H3] (STJ99156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Transfected

£149.00 - £360.00

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Anti-AKT1 antibody [3B4] (STJA0032625)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Monkey/Mouse/Rat

£224.50 - £308.50

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