Immune System | Research Area | St John's Laboratory

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FAK (Phospho-Tyr577) Antibody

Anti-Phospho-PTK2-Tyr577 antibody (543-592 aa) (STJ91185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-FAK (Y861) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTK2-Tyr861 antibody (828-877 aa) (STJ90268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat and HepG2 cells, using FAK (Phospho-Tyr925) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Tyr925 antibody (892-941 aa) (STJ90269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A-549 cells, using Phospho-FAK-Y397 Rabbit pAb (STJ27521) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PTK2-Y397 antibody (STJ27521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of A-549, using Phospho-FAK-Y397 Rabbit monoclonal antibody (STJ11105895) at 1:4000 dilution. A-549 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PTK2-Y397 antibody [S5895RM] (STJ11105895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates, using Phospho-FAK-Y576/577 Rabbit pAb (STJ117876) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 Mu g/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes. Raji cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PTK2-Y576/577 antibody (STJ117876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-FAK-Y925 Rabbit pAb (STJ29365) 1:1000 dilution.. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PTK2-Y925 antibody (STJ29365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-PTK2B-Tyr-402 antibody [M269] (STJA0005556)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PYK2 (Phospho-Tyr402) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2B-Tyr402 antibody (369-418 aa) (STJ91282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-PYK2 (Y579) Polyclonal Antibody

Anti-Phospho-PTK2B-Tyr579 antibody (545-594 aa) (STJ90970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of K562 cells using Phospho-PYK2 (Y580) Polyclonal Antibody

Anti-Phospho-PTK2B-Tyr580 antibody (546-595 aa) (STJ90770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of mouse cells using Phospho-PYK2 (Y881) Polyclonal Antibody

Anti-Phospho-PTK2B-Tyr881 antibody (847-896 aa) (STJ90769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (STJ22357) at 1:1000 dilution. Jurkat cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PTK2B-Y402 antibody (STJ22357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-PTPN1-Ser-50 antibody (STJA0005553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of COLO cells using Phospho-PTP1B (S50) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PTPN1-Ser50 antibody (16-65 aa) (STJ90882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-Phospho-PTPN1-Tyr-152 antibody (STJA0005554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various cells using Phospho-SH-PTP2 (Y542) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTPN11-Tyr542 antibody (508-557 aa) (STJ90741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-SH-PTP2 (Y580) Polyclonal Antibody

Anti-Phospho-PTPN11-Tyr580 antibody (546-595 aa) (STJ90742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SHP2-Y542 Rabbit pAb (STJ22358) at 1:700 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PTPN11-Y542 antibody (STJ22358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-PTPN6-Ser-591 antibody (STJA0005582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PTPN6-Tyr-536 antibody (STJA0005581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of RAW264.7+EGF cells using Phospho-SH-PTP1 (Y536) Polyclonal Antibody

Anti-Phospho-PTPN6-Tyr536 antibody (502-551 aa) (STJ90740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using SHP-1 (Phospho-Tyr564) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTPN6-Tyr564 antibody (530-579 aa) (STJ90575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Monkey

£49.00 - £261.00

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Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-SHP1-Y536 antibody (STJ22360).

Anti-Phospho-PTPN6-Y536 antibody (STJ22360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa and RAW 264. 7 cells, using Phospho-SHP1-Y564 Rabbit pAb (STJ117885) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour. RAW 264. 7 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-PTPN6-Y564 antibody (STJ117885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Immunofluorescence analysis of HeLa cells, using CD45 (Phospho-Ser1007) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PTPRC-Ser1007 antibody (981-1030 aa) (STJ90703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (STJ22364) at 1:1000 dilution. A-431 cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-RAC1-S71 antibody (STJ22364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human placenta, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RACGAP1-Ser387 antibody (353-402 aa) (STJ91093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (STJ113482) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RAF1-S259 antibody (STJ113482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb (STJ11102598) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RAF1-S259 antibody [S8MR] (STJ11102598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Raf1-S338 pAb (STJ117871) at 1:1000 dilution or Phospho-Raf1-S338 antibody (STJ25284). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-RAF1-S338 antibody (STJ117871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa and 293T cells, using Phospho-Raf1-S621 Rabbit pAb (STJ22366) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes or treated by EGF (25 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RAF1-S621 antibody (STJ22366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from C6 cells, using Phospho-Raf1-S621 Rabbit mAb (STJ11102597) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-RAF1-S621 antibody [S7MR] (STJ11102597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Anti-Phospho-RAF1-Ser-471 [S578/S433] antibody (STJA0005562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various cells using Phospho-Raf-1 (S259) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-RAF1-Ser259 antibody (225-274 aa) (STJ90399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Ser289 antibody (251-300 aa) (STJ91218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Raf-1 (S296) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RAF1-Ser296 antibody (271-320 aa) (STJ90850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RAF1-Ser301 antibody (STJ90595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Raf-1 (S338) Polyclonal Antibody

Anti-Phospho-RAF1-Ser338 antibody (305-354 aa) (STJ90676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using C-RAF (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Ser43 antibody (11-60 aa) (STJ90513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Ser621 antibody (591-640 aa) (STJ91052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of C-RAF (Phospho-Ser642) Antibody. The lane on the right is blocked with the C-RAF (Phospho-Ser642) peptide.

Anti-Phospho-RAF1-Ser642 antibody (599-648 aa) (STJ90659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using C-RAF (Phospho-Thr269) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Thr269 antibody (231-280 aa) (STJ91217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-RAF1-Tyr340 antibody (STJA0010022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using C-RAF (Phospho-Tyr341) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Tyr341 antibody (311-360 aa) (STJ90830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAPGEF1-Tyr504 antibody (470-519 aa) (STJ90537)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-REL-Ser492/460 antibody (STJA0010025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-c-Rel (S503) Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-REL-Ser503 antibody (470-519 aa) (STJ91012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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