• Western blot analysis of lysates from wild type (WT) and NDUFC2 knockout (KO) HeLa cells, using [KO Validated] NDUFC2 Rabbit polyclonal antibody (STJ117267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using [KO Validated] NDUFC2 Rabbit polyclonal antibody (STJ117267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NDUFC2 antibody (1-100) (STJ117267)

SKU:
STJ117267

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFC2 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFC2
Gene ID: 4718
Uniprot ID: NDUC2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
Immunogen Sequence: MIARRNPEPLRFLPDEARSL PPPKLTDPRLLYIGFLGYCS GLIDNLIRRRPIATAGLHRQ LLYITAFFFAGYYLVKREDY LYAVRDREMFGYMKLHPEDF
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis but required for the complex assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Subunit C2
Complex I-B14.5b
Ci-B14.5b
Human Lung Cancer Oncogene 1 Protein
Hlc-1
Nadh-Ubiquinone Oxidoreductase Subunit B14.5b
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6798695
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Subunit C2 antibody
Anti-Complex I-B14.5b antibody
Anti-Ci-B14.5b antibody
Anti-Human Lung Cancer Oncogene 1 Protein antibody
Anti-Hlc-1 antibody
Anti-Nadh-Ubiquinone Oxidoreductase Subunit B14.5b antibody
Anti-NDUFC2 antibody
Anti-HLC1 antibody

Information sourced from Uniprot.org

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