Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of L929 cells using SGK1 Rabbit polyclonal antibody (STJ25513) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGK1 antibody (172-431) (STJ25513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using SGK1 Mouse mAb diluted at 1:200.

Anti-SGK1 antibody (350-430 aa) [4D12] (STJ97721)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, SGK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SGK1 antibody (381-430 aa) (STJ95633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-SGK1 primary antibody (STJ502978) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 10X.

Anti-SGK1 antibody (40-90) (STJ502978)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£427.50

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Anti-SGK1 antibody (40-90) {Biotin} (STJ502979)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-SGK1 antibody (40-90) {FITC} (STJ502980)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-SHFM1 antibody (1-70 aa) (STJ11106444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-SHP2 antibody [SIT-01] (STJ16100481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Human

£485.50

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Western blot analysis of extracts of various cell lines, using Sin3A antibody (STJ11104619) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SIN3A antibody (1-100) [S4619RM] (STJ11104619)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using Sin3A antibody (STJ11104830) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SIN3A antibody (1-100) [S4830RM] (STJ11104830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of 293T cells, using Sin3A antibody (STJ115863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SIN3A antibody (1-120) (STJ115863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g Sin3A antibody (STJ25530). Western blot was performed from the immunoprecipitate using Sin3A antibody (STJ25530) at a dilution of 1:1000.

Anti-SIN3A antibody (1-120) (STJ25530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SIN3A antibody (STJA0007718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using SIN3B Rabbit pAb (STJ112598) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-SIN3B antibody (1-160) (STJ112598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Sin3B Polyclonal Antibody

Anti-SIN3B antibody (221-270 aa) (STJ94263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, SIRT1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SIRT1 antibody (13-62 aa) (STJ95667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using SIRT1 Rabbit polyclonal antibody (STJ11104332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SIRT1 antibody (200-300) (STJ11104332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (STJ25533) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT1 antibody (229-246) (STJ25533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human colon using SIRT1 Rabbit polyclonal antibody (STJ113751) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SIRT1 antibody (448-747) (STJ113751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SIRT1 antibody (448-747) (STJ119437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SIRT1 antibody (STJA0009872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis using SIRT1 monoclonal antibody against MCF-7 (1) , Jurkat (2) , HeLa (3) , HEK293 (4) and A549 (5) cell lysate.

Anti-SIRT1 antibody [1F3] (STJ98380)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot detection of SIRT1 in Hela, Jurkat, Ramos, A549 and CEM cell lysates using SIRT1 mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.

Anti-SIRT1 antibody [2G10-F4-D9] (STJ99238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of C6 cells using SIRT3 antibody (STJ11105707) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-SIRT3 antibody (20-130) (STJ11105707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SIRT3 Rabbit polyclonal antibody (STJ11102849) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT3 antibody (299-399) (STJ11102849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using SIRT3 Rabbit polyclonal antibody (STJ11104331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SIRT3 antibody (299-399) (STJ11104331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of HepG2 cells, using SIRT3 antibody (STJ28285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SIRT3 antibody (299-399) (STJ28285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts from wild type (WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody (STJ11104834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-SIRT3 antibody (300-399) [S4834RM] (STJ11104834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of the lysates from HepG2 cells using SIRT3 antibody.

Anti-SIRT3 antibody (350-399 aa) (STJ95669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SIRT3 Rabbit pAb (STJ29446) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-SIRT3 antibody (388-399) (STJ29446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of mouse fetal muscle, using SKI antibody (STJ11100892). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SKI antibody (700-800) (STJ11100892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SKI antibody (STJ192045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-SKIC8 antibody (STJA0008416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using SKIL antibody (STJ116284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SKIL antibody (350-450) (STJ116284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using SnoN Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SKIL antibody (616-665 aa) (STJ95721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using SKP1 antibody (STJ25541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SKP1 antibody (1-163) (STJ25541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Skp1 p19 Polyclonal Antibody

Anti-SKP1 antibody (41-90 aa) (STJ95675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various lysates using SKP1 Rabbit mAb (STJ11102409) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SKP1 antibody (64-163) [S9MR] (STJ11102409)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of C6 cells using SKP2 Rabbit polyclonal antibody (STJ116316) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SKP2 antibody (1-100) (STJ116316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of SKP2 in paraffin-embedded human colon using SKP2 Rabbit monoclonal antibody (STJ11101985) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SKP2 antibody (1-100) [S5MR] (STJ11101985)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis using Skp2 p45 monoclonal antibody against truncated Trx-Skp2 p45 recombinant protein (1) and GST-Skp2 p45 (aa1-130) recombinant protein (2).

Anti-SKP2 antibody (1-130 aa) [6G9D10] (STJ98381)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of U2OS cells using SKP2 antibody (STJ25542) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SKP2 antibody (100-200) (STJ25542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Skp2 p45 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SKP2 antibody (366-415 aa) (STJ95676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-SKP2 antibody [SKP2-8D9] (STJ16100869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/ELISA/IP

Reactivity :

Human/Non-Human Primates

£520.50

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Immunohistochemistry analysis of SLBP in paraffin-embedded human esophageal using SLBP Rabbit polyclonal antibody (STJ11103209) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-SLBP antibody (125-223) (STJ11103209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SLBP in 293T, Jurkat, Ramos lysates using SLBP antibody.

Anti-SLBP antibody (229-242 aa) [R04-1K2] (STJA0011969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£264.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g GLUT3/SLC2A3 antibody (STJ11102002). Western blot was performed from the immunoprecipitate using GLUT3/SLC2A3 antibody (STJ11102002) at a dilution of 1:1000.

Anti-SLC2A3 antibody (1-100) [S2MR] (STJ11102002)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from HepG2 cells, using GLUT3/SLC2A3 Rabbit pAb (STJ110449) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SLC2A3 antibody (207-281) (STJ110449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options