Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using PIWIL4 Rabbit pAb (STJ11107396) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-PIWIL4 antibody (STJ11107396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-PIWIL4 antibody [SRM14704] (STJ11109434)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates, using PKA C-alpha (PRKACA) antibody (STJ11102940) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PKA C-alpha antibody [S0MR] (STJ11102940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-PKC-beta antibody [SRM14455] (STJ11109185)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various cells using ZAC1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PLAGL1 antibody (311-360 aa) (STJ96292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-PLAGL1 antibody [SAA1920] (STJA0020348)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PLD6 antibody (29-79 aa) (STJ195220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin A Rabbit monoclonal antibody (STJ11102149, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PLIN1 antibody (400-500) [S9MR] (STJ11102149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Anti-PLIN1 antibody (STJA0009775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of C6 cells using Perilipin-2 Rabbit polyclonal antibody (STJ28198) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PLIN2 antibody (168-437) (STJ28198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western Blot analysis of varius cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-PLIN2 antibody (STJ192987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-PLIN4 antibody (STJA0007777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Snk Polyclonal Antibody

Anti-PLK2 antibody (291-340 aa) (STJ95720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PLK2 antibody (STJ29146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLK2 antibody (361-439) (STJ29146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Anti-PLK2 antibody (377-451 aa) (STJ98963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Fnk Polyclonal Antibody

Anti-PLK3 antibody (231-280 aa) (STJ93099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using PLK3 Rabbit pAb (STJ111375) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-PLK3 antibody (487-646) (STJ111375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PLXNA4 antibody (STJ193085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using PMAIP1 Rabbit pAb (STJ111843) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-PMAIP1 antibody (1-54) (STJ111843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PMAIP1 antibody (STJA0009777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PML antibody (STJ11101744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PML antibody (1-100) [S4MR] (STJ11101744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various cells using PML Polyclonal Antibody diluted at 1：1000

Anti-PML antibody (11-60 aa) (STJ95166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (STJ25034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PML antibody (300-600) (STJ25034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using PML antibody (STJ11100142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PML antibody (571-611) (STJ11100142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PML antibody [SRM15011] (STJ11109741)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/IHC-P/ELISA

Reactivity :

Human

£0.00

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Formalin-fixed, paraffin-embedded human colon adenocarcinoma (Lynch Syndrome) stained with anti-PMS-2 antibody. Note tumor cells are negative whereas normal glands (top) are nuclear positive.

Anti-PMS-2 antibody [ZR317] (STJ180591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£199.50 - £755.50

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Western blot analysis of various lysates using PMS2 Rabbit monoclonal antibody (STJ11102108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PMS2 antibody (1-100) [S8MR] (STJ11102108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates using PMS2 Rabbit polyclonal antibody (STJ29027) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PMS2 antibody (10-21) (STJ29027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Immunohistochemistry of mouse kidney with Anti-PMS2 primary antibody (STJ502561) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-PMS2 antibody (220-270) (STJ502561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PMS2 antibody (220-270) {Biotin} (STJ502563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PMS2 antibody (220-270) {FITC} (STJ502564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using PMS2 antibody (STJ115635) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PMS2 antibody (390-670) (STJ115635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of various cells using PMS2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PMS2 antibody (461-510 aa) (STJ95169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-PMS2antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 96 kDaObserved band size: 110 kDa

Anti-PMS2 antibody (600-700 aa) [PT2116] (STJ196728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-PMS2 antibody [EPR3947] (STJ16101659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£1,332.50

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Anti-PMS2 antibody [MRQ-28] (STJ16101579)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,436.50

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Anti-PMS2 antibody [PT0045R] (STJA0039756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£285.00 - £540.00

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Western blot analysis of lysates from wild type (WT) and PMS2 knockout (KO) HeLa cells, using [KO Validated] PMS2 Rabbit monoclonal antibody (STJ11104811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PMS2 antibody [S4811RM] (STJ11104811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of PMS2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200, RT, 1h.

Anti-PMS2 antibody [SM0372] (STJA0010372)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-PMS2 antibody [SM5009] (STJA0005009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of various lysates using ATGL/PNPLA2 Rabbit monoclonal antibody (STJ11102265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PNPLA2 antibody (300-394) [S5MR] (STJ11102265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of U2OS cells using ATGL/PNPLA2 Rabbit polyclonal antibody (STJ28001) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PNPLA2 antibody (45-257) (STJ28001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PNPLA2 antibody (STJA0006777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-POLDIP3 antibody (348-397 aa) (STJ95174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-POLR1A antibody (STJ190759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-POLR1A antibody [SAA1537] (STJA0019965)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of various lysates using POLR1B Rabbit polyclonal antibody (STJ113935) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-POLR1B antibody (670-900) (STJ113935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-POLR1B antibody (Internal) (STJ95534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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