Anti-PLK2 antibody (361-439) (STJ29146)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PLK2 (361-439) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLK2 |
| Gene ID | 10769 |
| Uniprot ID | PLK2_HUMAN |
| Immunogen Region | 361-439 |
| Immunogen Sequence | KNFFKKAAAALFGGKKDKAR YIDTHNRVSKEDEDIYKLRH DLKKTSITQQPSKHRTDEEL QPPTTTVARSGTPAVENKQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 361-439 of human PLK2 (NP_006613.2). |
Additional Info
| Tissue Specificity | Expressed at higher level in the fetal lung, kidney, spleen and heart. |
| Post Translational Modifications | Catalytic activity is enhanced by phosphorylation of Thr-239. |
| Function | Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CPAP, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CPAP and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress. |
| Protein Name | Serine/Threonine-Protein Kinase Plk2Polo-Like Kinase 2Plk-2Hplk2Serine/Threonine-Protein Kinase SnkHsnkSerum-Inducible Kinase |
| Database Links | Reactome: R-HSA-6804115Reactome: R-HSA-9662834Reactome: R-HSA-9768919 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCell ProjectionDendriteLocalizes To Centrosomes During Early G1 Phase Where It Only Associates To The Mother Centriole And Then Distributes Equally To Both Mother And Daughter Centrioles At The Onset Of S Phase |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Plk2 antibodyAnti-Polo-Like Kinase 2 antibodyAnti-Plk-2 antibodyAnti-Hplk2 antibodyAnti-Serine/Threonine-Protein Kinase Snk antibodyAnti-Hsnk antibodyAnti-Serum-Inducible Kinase antibodyAnti-PLK2 antibodyAnti-SNK antibody |
Information sourced from Uniprot.org