• Western blot analysis of various lysates using ATGL/PNPLA2 Rabbit monoclonal antibody (STJ11102265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PNPLA2 antibody (300-394) [S5MR] (STJ11102265)

SKU:
STJ11102265

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATGL/PNPLA2 (300-394) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNPLA2
Gene ID: 57104
Uniprot ID: PLPL2_HUMAN
Immunogen Region: 300-394
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-394 of human ATGL/PNPLA2 (NP_065109.1).
Immunogen Sequence: LPARLNEALLEACVEPTDLL TTLSNMLPVRLATAMMVPYT LPLESALSFTIRLLEWLPDV PEDIRWMKEQTGSICQYLVM RAKRKLGRHLPSRLP
Tissue Specificity Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
Post Translational Modifications Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity. Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation. Ubiquitinated by PEX2 in response to reactive oxygen species (ROS), leading to its degradation.
Function Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Also possesses acylglycerol transacylase and phospholipase A2 activities. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes.
Protein Name Patatin-Like Phospholipase Domain-Containing Protein 2
Adipose Triglyceride Lipase
Calcium-Independent Phospholipase A2-Zeta
Ipla2-Zeta
Desnutrin
Pigment Epithelium-Derived Factor Receptor
Pedf-R
Tts2.2
Transport-Secretion Protein 2
Tts2
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Lipid Droplet
Cell Membrane
Multi-Pass Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Patatin-Like Phospholipase Domain-Containing Protein 2 antibody
Anti-Adipose Triglyceride Lipase antibody
Anti-Calcium-Independent Phospholipase A2-Zeta antibody
Anti-Ipla2-Zeta antibody
Anti-Desnutrin antibody
Anti-Pigment Epithelium-Derived Factor Receptor antibody
Anti-Pedf-R antibody
Anti-Tts2.2 antibody
Anti-Transport-Secretion Protein 2 antibody
Anti-Tts2 antibody
Anti-PNPLA2 antibody
Anti-ATGL antibody
Anti-FP17548 antibody

Information sourced from Uniprot.org

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