Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of extracts of U-87MG cells, using PI3 Kinase p85 alpha antibody (STJ113826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R1 antibody (240-454) (STJ113826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (STJ113725) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PIK3R1 antibody (240-724) (STJ113725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-PIK3R1 antibody (550-630 aa) (STJ99654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Western blot analysis of various cells using PI 3-kinase p85 Alpha Polyclonal Antibody diluted at 1：1000

Anti-PIK3R1 antibody (573-622 aa) (STJ95077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody, and DAPI (blue)

Anti-PIK3R1 antibody (601-619 aa) [R03-3C7] (STJA0013015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£264.50

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Anti-PIK3R1 antibody (C-Term) [M253] (STJA0005530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£394.50

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Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using PI3 Kinase P85 Rabbit pAb diluted at 1:500.

Anti-PIK3R1 antibody (STJ197340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 1) 3T3, 2) Rat LiverTissue with PI3 Kinase P85 Alpha Mouse mAb diluted at 1:2, 000.

Anti-PIK3R1 antibody [3B7] (STJ97521)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-testis, antibody was diluted at 1:100

Anti-PIK3R2 antibody (1-60 aa) (STJ98987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 using PI3 Kinase p85 beta Rabbit polyclonal antibody (STJ119550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PIK3R2 antibody (1-85) (STJ119550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 beta antibody (STJ11102171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R2 antibody (100-200) [S1MR] (STJ11102171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of PI3 Kinase p85 beta in paraffin-embedded mouse spleen using PI3 Kinase p85 beta Rabbit polyclonal antibody (STJ119610) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PIK3R2 antibody (108-298) (STJ119610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PI 3 Kinase p85 beta antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PIK3R2 antibody (140-164 aa) [R06-5H3] (STJA0014501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Rat

£264.50

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Western blot analysis of various cells using PI 3-kinase p85 Beta Polyclonal Antibody diluted at 1：500

Anti-PIK3R2 antibody (409-458 aa) (STJ95080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of PI3 Kinase p85 beta in C6, K562, MCF7, U87MG, HCT116, 3T3 and Hela cell lysates using PI3 Kinase p85 beta mouse mAb (1:1000 diluted).Predicted band size:85KDa.Observed band size:85KDa.

Anti-PIK3R2 antibody [8D9-D5-F8] (STJ99192)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of various lysates using PIK3R3/p55PIK Rabbit pAb (STJ110251) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PIK3R3 antibody (1-230) (STJ110251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using PIK3R3/p55PIK Rabbit polyclonal antibody (STJ11101208) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PIK3R3 antibody (300-388) (STJ11101208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PIK3R3 antibody (300-388) (STJ119452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PIK3R3/p55PIK Rabbit monoclonal antibody (STJ11101979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIK3R3 antibody (362-461) [S9MR] (STJ11101979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PIK3R3 antibody (STJ191947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using PIPK I Gamma Polyclonal Antibody

Anti-PIP5K1C antibody (305-354 aa) (STJ95100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-PIP5K1C antibody (450-550 aa) (STJ11106390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PITPNA antibody (160-240 aa) (STJ95102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using PITPNA Rabbit polyclonal antibody (STJ114832) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PITPNA antibody (211-270) (STJ114832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PKA C-alpha (PRKACA) antibody (STJ11102940) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PKA C-alpha antibody [S0MR] (STJ11102940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-PKC alpha antibody [SRM] (STJ11106955)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of 293T cells, using PKNOX1 antibody (STJ117496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PKNOX1 antibody (322-436) (STJ117496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PKNOX1 antibody (STJ191935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PKP2 antibody (STJ27458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PKP2 antibody (578-837) (STJ27458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Plakophilin 2 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PKP2 antibody (632-681 aa) (STJ95149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-PKP3 antibody (1-220 aa) (STJ11107470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-PKP3 antibody (STJ192943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of mouse-heart lysis using Plakophilin 4 antibody. Antibody was diluted at 1:2000

Anti-PKP4 antibody (185-234 aa) (STJ95150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PLAC8 antibody (STJA0007266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1) ) antibody (STJ118150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PLCG1 antibody (1202-1291) (STJ118150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1) ) antibody (STJ110022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PLCG1 antibody (700-800) (STJ110022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PLC gamma 1 (PLC gamma 1 (PLCG1) ) Rabbit mAb (STJ11102392) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PLCG1 antibody (710-832) [S2MR] (STJ11102392)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PLC Gamma 1 Polyclonal Antibody diluted at 1：2000

Anti-PLCG1 antibody (736-785 aa) (STJ95157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-PLCG1 antibody (N-Term) [M156] (STJA0005536)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

£394.50

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Western blot analysis of HepG2, using PerilipinA antibody (STJ118726) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PLIN1 antibody (400-500) (STJ118726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin A Rabbit monoclonal antibody (STJ11102149, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PLIN1 antibody (400-500) [S9MR] (STJ11102149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Perilipin A in Human fetal liver lysates using Perilipin 1 antibody.

Anti-PLIN1 antibody (475-500 aa) [R08-9B4] (STJA0014403)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£264.50

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Anti-PLIN1 antibody (STJA0009775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Plexin A1 Rabbit monoclonal antibody (STJ11101931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PLXNA1 antibody (100-200) [S1MR] (STJ11101931)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PLXNA1 antibody (Sema Domain) (STJA0005538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXNA1 antibody (Sema Domain) (STJA0005540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXNA1 antibody (Sema Domain) [M535] (STJA0005539)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£394.50

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Anti-PLXNA1 antibody (STJA0009804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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