• Western blot analysis of extracts of various cell lines, using Integrin alpha 6 (ITGA6/CD49f) antibody (STJ11103465) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Integrin alpha 6 (ITGA6/CD49f) antibody (STJ11103465) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Integrin alpha 6 antibody (481-591) [S5MR] (STJ11103465)

SKU:
STJ11103465

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Integrin alpha 6 (ITGA6/CD49f) (481-591) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA6
Gene ID: 3655
Uniprot ID: ITA6_HUMAN
Immunogen Region: 481-591
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 481-591 of human Integrin alpha 6 (ITGA6/CD49f) (NP_001073286.1).
Immunogen Sequence: IDLRQKTACGAPSGICLQVK SCFEYTANPAGYNPSISIVG TLEAEKERRKSGLSSRVQFR NQGSEPKYTQELTLKRQKQK VCMEETLWLQDNIRDKLRPI PITASVEIQEP
Tissue Specificity Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines.they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Processed integrin alpha-6: Expressed at low levels in normal prostate tissue with elevated levels in prostate cancer tissue (at protein level).
Post Translational Modifications Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand. Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p). Production of alpha6p enhances prostate cancer cell invasion and migration. Palmitoylation by DHHC3 enhances stability and cell surface expression.
Function Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
Protein Name Integrin Alpha-6
Cd49 Antigen-Like Family Member F
Vla-6
Cd Antigen Cd49f Cleaved Into - Integrin Alpha-6 Heavy Chain - Integrin Alpha-6 Light Chain - Processed Integrin Alpha-6
Alpha6p
Database Links Reactome: R-HSA-2022090
Reactome: R-HSA-210991
Reactome: R-HSA-216083
Reactome: R-HSA-3000157
Reactome: R-HSA-3000170
Reactome: R-HSA-446107
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Lipid-Anchor
Alternative Antibody Names Anti-Integrin Alpha-6 antibody
Anti-Cd49 Antigen-Like Family Member F antibody
Anti-Vla-6 antibody
Anti-Cd Antigen Cd49f Cleaved Into - Integrin Alpha-6 Heavy Chain - Integrin Alpha-6 Light Chain - Processed Integrin Alpha-6 antibody
Anti-Alpha6p antibody
Anti-ITGA6 antibody

Information sourced from Uniprot.org

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