Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using p38 MAPK rabbit monoclonal antibody (STJ11102152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-MAPK14 antibody (100-200) [S2MR] (STJ11102152)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, p38 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-MAPK14 antibody (147-196 aa) (STJ94878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish/Pig

£48.00 - £252.00

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Immunohistochemistry with Anti-MAPK14 Antibody (STJ501693) at 1:100 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000. FFPE section.

Anti-MAPK14 antibody (150-200) (STJ501693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK14 antibody (150-200) {Biotin} (STJ501694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK14 antibody (150-200) {FITC} (STJ501695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (STJ112728) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-MAPK14 antibody (261-360) (STJ112728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £642.50

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Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (STJ113086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK14 antibody (261-360) (STJ113086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells, using 3 Mu g p38 MAPK antibody (STJ116613). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (STJ116613) at a dilution of 1:1000.

Anti-MAPK14 antibody (261-360) (STJ116613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using p38 MAPK Rabbit polyclonal antibody (STJ27472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAPK14 antibody (261-360) (STJ27472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates of HepG2 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p38 (PTR2306) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 41kDaObserved band size: 38kDa

Anti-MAPK14 antibody (300-360 aa) [4G2] (STJ196672)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of HELA KB PC-3 using p38 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-MAPK14 antibody (C-Term) (STJ97615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Anti-Mapk14 antibody (C-Term) [M548] (STJA0005504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-MAPK14 antibody (STJ99619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using ERK1 Rabbit monoclonal antibody (STJ11104593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MAPK3 antibody (1-100) [S4593RM] (STJ11104593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

£129.50 - £574.50

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Western blot with Anti-MAPK3 Antibody (STJ501702) of rat testis at 1:250 dilution in 1% milk solution.

Anti-MAPK3 antibody (300-350) (STJ501702)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK3 antibody (300-350) {Biotin} (STJ501703)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK3 antibody (300-350) {FITC} (STJ501704)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ERK 1 monoclonal antibody against HeLa (1) , Jurkat (2) , RAW264.7 (3) , HEK293 (4) , K562 (5) , NIH/3T3 (6) , Cos7 (7) and PC-12 (8) cell lysate.

Anti-MAPK3 antibody [1E5] (STJ98045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of rat-spleen tissue. 1, ERK1 Mouse monoclonal antibody (5E9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MAPK3 antibody [5E9] (STJ97780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of MCF-7 cells using ERK5 antibody (STJ24489). Blue: DAPI for nuclear staining.

Anti-MAPK7 antibody (1-815) (STJ24489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MAPK7 antibody (700-750) (STJ501711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK7 antibody (700-750) {Biotin} (STJ501712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK7 antibody (700-750) {FITC} (STJ501713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using ERK5 Rabbit monoclonal antibody (STJ11101971) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-MAPK7 antibody (717-816) [S1MR] (STJ11101971)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Skeletal Muscle, 4) Rat Kidney, 5) Rat Skeletal Muscle using ERK 5 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-MAPK7 antibody (STJ197395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using MAPK8 Rabbit polyclonal antibody (STJ11105575) at 1:400 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-MAPK8 antibody (1-100) (STJ11105575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using JNK1 antibody (STJ24490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK8 antibody (245-345) (STJ24490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using JNK1 Rabbit polyclonal antibody (STJ24491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK8 antibody (245-345) (STJ24491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of JNK1 in C6, 3T3, Hela lysates using JNK1 antibody.

Anti-MAPK8 antibody (325-343 aa) [R08-7H1] (STJA0011517)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£246.50

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Immunohistochemistry Colon with Anti-MAPK8 Antibody (STJ501714) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 5X magnification on Leica DM4000. FFPE section.

Anti-MAPK8 antibody (370-420) (STJ501714)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-MAPK8 antibody (370-420) {Biotin} (STJ501715)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-MAPK8 antibody (370-420) {FITC} (STJ501716)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-MAPK8 antibody (C-Term) [M267] (STJA0005455)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46, 54KDa.Observed band size:46KDa.

Anti-MAPK8 antibody [1A4-C5-F11] (STJ99225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Anti-Matrix Metalloproteinase-2 antibody [4D3] (STJ16100697)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/WB

Reactivity :

Human

£771.50

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Anti-MBP antibody (100-140 aa) (STJ13101368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Human cerebrum tissue was stained with Anti-Myelin Basic Protein (MBP) (ABT-MBP) Antibody

Anti-MBP antibody (150-250 aa) [ABT-MBP] (STJ196930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Confocal imaging of paraffin-embedded Mouse brain using Myelin Basic Protein Rabbit monoclonal antibody (STJ11101285, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.

Anti-MBP antibody (161-304) [S5MR] (STJ11101285)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MBP antibody (180-260 aa) (STJ190136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-MBP antibody (2-40 aa) (STJ13101425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various cell lines, using Myelin Basic Protein antibody (STJ24515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MBP antibody (80-165) (STJ24515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MBP antibody (N-Term) (STJA0005812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-MBP antibody (STJ13100186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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