Developmental Biology | Research Area | St John's Laboratory

Western blot analysis using Nkx-2.5 monoclonal antibody against full-length NKX2.5 (aa1-324) -hIgGFc transfected HEK293 cell lysate (1).

Anti-NKX2-5 antibody [2E1] (STJ98278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Human Ewing NA s sarcoma stained with anti-NKX2. 2 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Anti-NKX2.2 antibody (1-119aa) [ZM14] (STJ180293)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£164.50 - £601.50

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Immunohistochemistry analysis of paraffin-embedded mouse pancreas using NKX6-1 antibody (STJ11103302) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NKX6-1 antibody (1-100) (STJ11103302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of NKX61 Antibody. The lane on the right is blocked with the NKX61 peptide.

Anti-NKX6-1 antibody (180-260) (STJ94508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NMDAR1 antibody (ECD) (STJ13100195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of extracts of various cell lines, using NODAL antibody (STJ111943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NODAL antibody (198-347) (STJ111943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of Hela lysis using NODAL antibody.. Secondary antibody was diluted at 1:20000

Anti-NODAL antibody (294-343 aa) (STJ97680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-NOG antibody (21-70 aa) (STJ98814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NOG antibody (STJ110603) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-NOG antibody (28-232) (STJ110603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-NOG antibody (Internal Region) (STJA0005795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Western blot detection of Noggin in CHO-K1 cell lysates over-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:37KDa.

Anti-NOG antibody [1D2-G8-G1] (STJ99187)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts of A-549 cells, using Non-phospho-YAP1-S127 antibody (STJ11103239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Non-phospho-YAP1-S127 antibody (50-150) (STJ11103239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Jurkat cells, using Notch1 antibody (STJ11100238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NOTCH1 antibody (1567-1729) (STJ11100238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Notch1 antibody (STJ119092) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (1715-1814) (STJ119092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using Notch1 Rabbit polyclonal antibody (STJ11103863) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH1 antibody (2381-2480) (STJ11103863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Rat brain cells, using Notch1 antibody (STJ29951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (2456-2555) (STJ29951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g Notch1 antibody (STJ11101627). Western blot was performed from the immunoprecipitate using Notch1 antibody (STJ11101627) at a dilution of 1:1000.

Anti-NOTCH1 antibody (2456-2555) [S7MR] (STJ11101627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of extracts of U-251MG cells, using Notch1 antibody (STJ119090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-NOTCH1 antibody (2504-2518) (STJ119090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NPNT antibody (485-535 aa) (STJ193586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using NR2F2 antibody (STJ112289) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR2F2 antibody (1-100) (STJ112289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using ARP-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NR2F2 antibody (1-50 aa) (STJ91700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Steroidogenic factor-1 (SF-1/NR5A1) Rabbit polyclonal antibody (STJ28057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR5A1 antibody (1-120) (STJ28057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from rat lung, using STF-1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NR5A1 antibody (169-218 aa) (STJ95626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Rat brain, using SF-1 Rabbit polyclonal antibody (STJ11105403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NR5A1 antibody (218-461) (STJ11105403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Human adrenal gland stained with anti-SF-1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of cortical cells.

Anti-NR5A1 antibody (220-461aa) [N1665] (STJ180239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£233.50 - £1,103.50

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Western blot analysis of GTPase HRAS in 3T3, mouse brain lysates using GTPase HRAS antibody.

Anti-NRAS antibody (27-40 aa) [R01-7D0] (STJA0012568)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Hamster

£264.50

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Anti-NRAS antibody (7-57) (STJA0006494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using NRAS Rabbit polyclonal antibody (STJ11103616) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NRAS antibody (90-189) (STJ11103616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NRCAM antibody (1050-1130 aa) (STJ192457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using NRCAM Rabbit polyclonal antibody (STJ11103191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NRCAM antibody (25-115) (STJ11103191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of A-549, using Neuropilin-1 (NRP1) antibody (STJ11104753) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:200000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-NRP1 antibody (22-644) [S4753RM] (STJ11104753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of 293 cells using Neuropilin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NRP1 antibody (321-370 aa) (STJ96817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Neuropilin-1 Polyclonal Antibody

Anti-NRP1 antibody (476-525) (STJ94438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Neuropilin-1 (NRP1) Rabbit monoclonal antibody (STJ11101624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NRP1 antibody (824-923) [S4MR] (STJ11101624)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of SH-SY5Y cells using Neuropilin-1 (NRP1) Rabbit polyclonal antibody (STJ119109) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NRP1 antibody (901-923) (STJ119109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NRP1 antibody (a1 CUB Domain) (STJA0005484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-NRP1 antibody (a1 CUB Domain) [M534] (STJA0005483)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£367.50

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Immunofluorescence analysis of PC-12 cells using NRP2 Rabbit polyclonal antibody (STJ24823) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NRP2 antibody (21-931) (STJ24823)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HepG2 cells using Neuropilin-2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NRP2 antibody (791-840 aa) (STJ96595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of K562, L929 cells using NTN Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-NRTN antibody (111-160 aa) (STJ97344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-NRTN antibody (C-Term) (STJ118837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot with Anti-NTN1 Antibody (STJ501984) and NTRN peptide. Antibody at 1:500 dilution in buffer.

Anti-NTN1 antibody (250-300) (STJ501984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-NTN1 antibody (250-300) {Biotin} (STJ501985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-NTN1 antibody (250-300) {FITC} (STJ501986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various cells using Netrin-1 Polyclonal Antibody diluted at 1：1000

Anti-NTN1 antibody (484-533) (STJ94407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NTN1 antibody (STJ118675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NTN1 antibody (500-600) (STJ118675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using NTN4 Rabbit polyclonal antibody (STJ115720) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NTN4 antibody (290-520) (STJ115720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NTN4 antibody (50-130 aa) (STJ192363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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