Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using E-Cadherin antibody (STJ113820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CDH1 antibody (700-800) (STJ113820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Human Breast carcinoma tissue was stained with E-cadherin (ABT181) Antibody

Anti-CDH1 antibody (700-800) [ABT181] (STJA0006294)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£144.00 - £234.00

Choose Options

Human Breast tissue was stained with E-cadherin (ABT270) Antibody

Anti-CDH1 antibody (700-800) [ABT270] (STJA0006293)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ELISA

Reactivity :

Human

£144.00 - £234.00

Choose Options

Anti-CDH1 antibody (C-Term) (STJA0005284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

Choose Options

Western blot detection of E-Cadherin in T47D, HCT116, MDA-MB-468 and Caco-2 cell lysates using E-Cadherin mouse mAb (dilution 1:2000).Predicted band size:135kDa.Observed band size:135kDa.

Anti-CDH1 antibody [6B10-F4-G10] (STJ99316)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis using E-cadherin monoclonal antibody against LNCAP (1) , A431 (2) , DU145 (3) , PC-3 (4) , PC-12 (5) and T47D (6) cell lysate.

Anti-CDH1 antibody [7A2] (STJ98010)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

Choose Options

Western blot analysis using E-cadherin monoclonal antibody against LNCAP (1) , A431 (2) , DU145 (3) , PC-3 (4) , MCF-7 (5) , PC-12 (6) , NIH/3T3 (7) , C6 (8) and COS7 (9) cell lysate.

Anti-CDH1 antibody [7H12] (STJ98009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

Choose Options

Western blot analysis of various cells using M-cadherin Polyclonal Antibody diluted at 1：500

Anti-CDH15 antibody (81-130 aa) (STJ94042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of extracts from wild type (WT) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (STJ11101620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-CDH2 antibody (100-200) [S0MR] (STJ11101620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-N-Cadherin (ABT-CDH2) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Hela Lane 3: A431Predicted band size: 100kDaObserved band size: 110kDa

Anti-CDH2 antibody (200-400 aa) [ABT-CDH2] (STJ196931)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

Choose Options

Anti-CDH2 antibody (200-400 aa) [PT0199] (STJ196852)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

Choose Options

Immunofluorescence analysis of paraffin-embedded mouse heart using N-Cadherin Rabbit polyclonal antibody (STJ23043). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CDH2 antibody (488-906) (STJ23043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using N-Cadherin Rabbit polyclonal antibody (STJ110955) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDH2 antibody (500-800) (STJ110955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of rat-lung tissue. 1, N-cadherin Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-CDH2 antibody (721-770) (STJ94353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g N-Cadherin antibody (STJ11104277). Western blot was performed from the immunoprecipitate using N-Cadherin antibody (STJ11104277) at a dilution of 1:1000.

Anti-CDH2 antibody (807-906) (STJ11104277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of mouse heart, using N-Cadherin antibody (STJ114884) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDH2 antibody (807-906) (STJ114884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Endogenous CDH2 detected with at 1:500 dilution lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CDH2 antibody (850aa to C-Term) (STJ140028)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

Choose Options

Anti-CDH2 antibody (C-Term) (STJA0005286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£319.50

Choose Options

Anti-CDH2 antibody (C-Term) (STJA0005289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

Choose Options

Anti-CDH2 antibody (Cytoplasmic) [M170] (STJA0005285)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£390.50

Choose Options

Anti-CDH2 antibody (STJA0005288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Western blot analysis using N-cadherin monoclonal antibody against A431 (1) , NIH/3T3 (2) , HeLa (3) , C6 (4) and LNCap (5) cell lysate.

Anti-CDH2 antibody [5D5] (STJ98267)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

Choose Options

Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-R-Cadherin (PT2127) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: Hela Lane 2: HepG2 Lane 3: HEK293Predicted band size:100kDaObserved band size: 120kDa

Anti-CDH4 antibody (150-250) [PT2127] (STJA0006237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of A549 cells, using CDH4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CDH4 antibody (731-780 aa) (STJ95395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using CDH4 Rabbit polyclonal antibody (STJ112575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDH4 antibody (757-916) (STJ112575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-CDK19 antibody (250-350 aa) (STJ11106292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of CDK19 in paraffin-embedded rat testis using CDK19 Rabbit polyclonal antibody (STJ118549) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK19 antibody (250-350) (STJ118549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-CDK19 antibody (30-110 aa) (STJ191675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using CDK2 rabbit monoclonal antibody (STJ11101225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK2 antibody (199-298) [S5MR] (STJ11101225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer using [KO Validated] CDK2 Rabbit polyclonal antibody (STJ113540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK2 antibody (200-298) (STJ113540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of various cell lines, using CDK2 rabbit polyclonal antibody (STJ119215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDK2 antibody (200-298) (STJ119215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using CDK2 antibody (STJ119973) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CDK2 antibody (200-298) (STJ119973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, Cdk2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK2 antibody (231-280 aa) (STJ92197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, Cdk4 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDK4 antibody (1-50 aa) (STJ96563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g CDK4 antibody (STJ11104280). Western blot was performed from the immunoprecipitate using CDK4 antibody (STJ11104280) at a dilution of 1:1000.

Anti-CDK4 antibody (200-303) (STJ11104280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of various cell lines, using CDK4 antibody (STJ23055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK4 antibody (200-303) (STJ23055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-CDK4 antibody (204-303 aa) [SRM] (STJ11106945)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.

Anti-CDK4 antibody (204-303) (STJ11101180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of various lysates, using CDK4 rabbit monoclonal antibody (STJ11101279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK4 antibody (204-303) [S9MR] (STJ11101279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£146.50 - £642.50

Choose Options

Western blot analysis using Cdk4 monoclonal antibody against HeLa, LNCAP, F9 cell lysate.

Anti-CDK4 antibody (STJ98476)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Dog/Pig

£144.00 - £348.00

Choose Options

Western blot detection of CDK4 in Hela, Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

Anti-CDK4 antibody [3F9-B12-C8] (STJ99110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

Choose Options

Anti-Cdk4 antibody [DCS-31] (STJ16100131)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IF/WB/FC/IP

Reactivity :

Human/Mouse/Rat

£977.50

Choose Options

Anti-CDK4 antibody [EP180] (STJ16101441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£1,412.50

Choose Options

Immunoprecipitation analysis of 900 Mu g extracts of A-549 cells using 3 Mu g CDK5 antibody (STJ28297). Western blot was performed from the immunoprecipitate using CDK5 antibody (STJ28297) at a dilution of 1:1000.

Anti-CDK5 antibody (1-292) (STJ28297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of HepG2 cells using Cdk5 Polyclonal Antibody diluted at 1：500

Anti-CDK5 antibody (1-50 aa) (STJ92200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells, using [KO Validated] CDK5 Rabbit polyclonal antibody (STJ11100053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK5 antibody (170-289) (STJ11100053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of Hela, using CDK5 rabbit monoclonal antibody (STJ11101567) at 1:1000 dilutionSecondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDK5 antibody (193-292) [S7MR] (STJ11101567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells, using [KO Validated] CDK5 Rabbit monoclonal antibody (STJ11105132) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK5 antibody (92-289 aa) [S5132RM] (STJ11105132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options