Cell Cycle | Research Area | St John's Laboratory

Anti-Phospho-p53-Ser6 antibody [PT1249R] (STJA0040157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Phospho-p53 Ser6 antibody western blot A549 MCF-7 STJA0035083

Anti-Phospho-p53-Ser6 antibody [R02-0F2] (STJA0035083)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Phospho-p53 Ser9 antibody western blot HepG2 HeLa STJA0035775

Anti-Phospho-p53-Ser9 antibody [R03-4Y8] (STJA0035775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-p53-T18 antibody [23#] (STJA0019168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Phospho-p53 Thr55 antibody western blot HEK293 STJA0035934

Anti-Phospho-p53-Thr55 antibody [R06-4R5] (STJA0035934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Phospho-p95 Ser343 antibody western blot Jurkat lysates STJA0035850

Anti-Phospho-p95-Ser343 antibody [R04-5S5] (STJA0035850)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-PKC Alpha-S657/Y658 antibody (STJA0041328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IP

Reactivity :

Human/Rabbit/Rat

£542.50

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Anti-Phospho-PKC alpha-Ser657 antibody [R05-8I3] (STJA0035085)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Anti-Phospho-PKC alpha-Thr638 antibody [R09-7C7] (STJA0033921)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Phospho-PKC alpha antibody western blot Jurkat lysate STJA0035672

Anti-Phospho-PKC alpha-Thr638 antibody [R84-8J-5] (STJA0035672)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYT1 (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PKMYT1-Ser83 antibody (49-98 aa) (STJ91212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of PLK1 (Phospho-Ser137) Antibody. The lane on the right was blocked with the PLK1 STJ90594

Anti-Phospho-PLK1-Ser137 antibody (103-152 aa) (STJ90594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HT-29 cells, using Phospho-PLK1-T210 (STJ11102606) at 1:1000 dilution. HT-29 cells were treated by nocodazole (100 ng/mL) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-PLK1-T210 antibody [S6MR] (STJ11102606)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of 293 using p-Plk (T210) antibody.

Anti-Phospho-PLK1-Thr210 antibody (176-225 aa) (STJ91124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-PLK1 antibody western blot HeLa cells STJA0036131

Anti-Phospho-PLK1-Thr210 antibody [R08-3V9] (STJA0036131)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-PLK4 antibody (300-350) (STJ506388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£497.50

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Anti-Phospho-PLK4 antibody (300-350) {Biotin} (STJ506389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£551.50

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Anti-Phospho-PLK4 antibody (300-350) {FITC} (STJ506390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£551.50

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Western blot PLK protein phosphorylated at Thr210 in rat synaptic membrane STJA0003749

Anti-Phospho-Polo-Like Kinase-Thr210 antibody (STJA0003749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Non-Human Primates/Mouse/Xenopus/Zebrafish

£488.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [1stS2] (STJA0019158)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [1stS5] (STJA0019159)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [2ndS2] (STJA0019161)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [S7] (STJA0019160)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [S7S5] (STJA0019162)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (STJ116378) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by Doxorubicin (0. 5uM) for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S2 antibody (STJ116378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (STJ11102585). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Phospho-POLR2A-S2 antibody [S5MR] (STJ11102585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of HeLa, using Phospho-POLR2A CTD-S2/S5 antibody (STJ11105825) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-Phospho-POLR2A-S2/S5 antibody (STJ11105825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit pAb (STJ117926) at 1:1000 dilution. MCF7 and C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody (STJ117926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (STJ11103724) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. MCF7 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S7 antibody (STJ11103724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-POLR2A-Ser1619 antibody (1585-1634 aa) (STJ91031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-Phospho-POLR2A-Ser2 antibody (STJ196405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-POLR2A-Ser5 antibody (STJ196406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-POLR2A-Ser7 antibody (STJ196407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Chromatin immunoprecipitation analysis of extracts of 293T; cells, using Phospho-POLR2A CTD-T4 antibody (STJ11104227) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Phospho-POLR2A-T4 antibody (STJ11104227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using Phospho-POLR2A-T4 monoclonal antibody antibody (STJ11105859) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-POLR2A-T4 antibody [S5859RM] (STJ11105859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-POLR2A-Thr4 antibody (STJ196408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-PP2A alpha antibody western blot rat kidney A431 STJA0035755

Anti-Phospho-PP2A alpha-Tyr307 antibody [R07-9C6] (STJA0035755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-S507 Rabbit pAb (STJ116389) at 1:2000 dilution or PPP1R12A antibody (STJ113547). HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PPP1R12A-S507 antibody (STJ116389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-S668 Rabbit pAb (STJ11100976) at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PPP1R12A-S668 antibody (STJ11100976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser507 antibody (STJ196361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser668 antibody (STJ196362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser668/Ser618 antibody (STJA0010031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using Phospho-PPP1R12A-T853 Rabbit pAb ( STJ11101051) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PPP1R12A-T853 antibody (STJ11101051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PPP1R12A-Thr696 antibody (661-710 aa) (STJ91291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of human-lung tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PPP1R12A-Thr853 antibody (621-670 aa) (STJ90822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, PP2A-C Alpha (phospho Tyr307) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-PPP2CA-Tyr307 antibody (260-309 aa) (STJ91049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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