Cell Cycle | Research Area | St John's Laboratory

Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3 Beta-Y216 + GSK3 Alpha-Y279 Rabbit pAb (STJ116370) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda PP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-GSK3B-Y216/Y279 antibody (STJ116370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-GSK3 Beta-Y216 + GSK3 Alpha-Y279 Rabbit pAb (STJ110852) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-GSK3B-Y279 antibody (STJ110852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-H2AX-Ser139 antibody (94-143 aa) (STJ90288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

£49.00 - £261.00

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Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-H2AX-Ser139 antibody (STJA0009882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£149.00 - £360.00

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Anti-Phospho-H2AX-Thr120 antibody (STJ197460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-H2AX-Tyr142 antibody (STJ197459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-H2BC3-Ser-36 antibody (STJA0005438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-H2BC9-Ser14 antibody (STJ194711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH 9.0 was used for antigen retrieval. 2, STJ91330

Anti-Phospho-H3-3A/H3-3B-Ser31 antibody (16-65 aa) (STJ91330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-H4C1-Tyr-72 antibody (STJA0005439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-HDAC1-Ser406 antibody [R06-1W-1] (STJA0033026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£224.50 - £319.50

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Western blot analysis of lysates from Jurkat cells treated with EGF 200 ng/ml for 30', using HDAC1 (Phospho-Ser421) STJ90497

Anti-Phospho-HDAC1-Ser421 antibody (387-436 aa) (STJ90497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (STJ27513) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-Histone H2A-S139 antibody (STJ27513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Phospho-Histone H2AX antibody western blot 3T3 cells STJA0033230

Anti-Phospho-Histone H2A.X-Ser139 antibody [2A9-B5] (STJA0033230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Histone H2AX Ser139 antibody western blot 3T3 STJA0033231

Anti-Phospho-Histone H2A.X-Ser139 antibody [7G9-H4] (STJA0033231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Histone H2AX antibody western blot Jurkat STJA0036230

Anti-Phospho-Histone H2A.X-Ser139 antibody [R03-2Z1] (STJA0036230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H2AX antibody western blot HepG2 STJA0034346

Anti-Phospho-Histone H2A.X-Ser139 antibody [R09-4A7] (STJA0034346)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot Histone H2B antibody detection in Jurkat cells with phosphatase treatment STJA0041244

Anti-Phospho-Histone H2B-Ser-36 antibody (STJA0041244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemistry of baboon spleen with Anti-Phospho-Histone H3 primary antibody (S10) (STJ502483) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-Phospho-Histone H3 antibody (1-50) (STJ502483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£497.50

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Anti-Phospho-Histone H3 antibody (1-50) {Biotin} (STJ502484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£551.50

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Anti-Phospho-Histone H3 antibody (1-50) {FITC} (STJ502485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£551.50

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Phospho-Histone H3 antibody immunohistochemistry gastric cancer STJA0037643

Anti-Phospho-Histone H3-Ser10 antibody [BP6092] (STJA0037643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Phospho-Histone H3 antibody western blot HeLa STJA0035817

Anti-Phospho-Histone H3-Ser10 antibody [R06-6O9] (STJA0035817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H3 antibody immunocytochemistry HeLa cells STJA0035070

Anti-Phospho-Histone H3-Ser10/Thr11 antibody [R08-6C4] (STJA0035070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H3 Ser28 antibody western blot 3T3 HeLa STJA0034415

Anti-Phospho-Histone H3-Ser28 antibody [R03-7K5] (STJA0034415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-Histone H3-T11 antibody [N123/19] (STJA0019175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£195.50 - £1,319.50

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Phospho-Histone H3 Thr3 antibody western blot C6 3T3 HeLa STJA0033904

Anti-Phospho-Histone H3-Thr3 antibody [R08-9D6] (STJA0033904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H3 Thr3 antibody western blot HeLa STJA0035656

Anti-Phospho-Histone H3-Thr3 antibody [R21-3K-2] (STJA0035656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Histone H3 (Phospho-Thr3) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Histone H3.1-Thr3 antibody (1-50 aa) (STJ90291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Histone H3.3-S31 antibody [SRM13685] (STJ11108415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (STJ11101036) at 1:1000 dilution or Histone H4 Rabbit pAb (STJ29382). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-Histone H4-S1 antibody (STJ11101036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Histone H4-Ser1 antibody (STJ197451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C overnight STJ91343

Anti-Phospho-Histone H4-Ser47 antibody (13-62 aa) (STJ91343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Histone H4-Thr80 antibody (STJ197450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot Histone H4 Tyr-72 phosphorylation in treated and untreated PC3 cells STJA0041245

Anti-Phospho-Histone H4-Tyr-72 antibody (STJA0041245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Anti-Phospho-HSP90AA1-Thr5/7 antibody (STJ196324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, HSP90 Beta (phospho Ser226) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-HSP90AB1-Ser226 antibody (201-250 aa) (STJ90725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HSP90 Beta (S254) Polyclonal Antibody

Anti-Phospho-HSP90AB1-Ser254 antibody (226-275 aa) (STJ91017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-INCENP-Thr59 antibody (STJA0009973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-IRS1-Ser612 antibody (STJ196336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry of mouse small intestine with Anti-Phospho-JAK2 primary antibody (STJ502510) at 1:50 dilution in buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-Phospho-JAK2 antibody (980-1030) (STJ502510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Phospho-JAK2 antibody (980-1030) {Biotin} (STJ502511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-JAK2 antibody (980-1030) {FITC} (STJ502512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-JAK2-Tyr1007 antibody (981-1030 aa) (STJ90315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-JAK2 antibody western blot MEF C6 Pervanadate STJA0036229

Anti-Phospho-JAK2-Tyr1007/1008 antibody [R02-1C5] (STJA0036229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-JAK2-Tyr1008 antibody (STJ196340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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