Rat sCD14 (sCD14) Antibody Pair (STJAP00938)
General Information
| Short Description | This sCD14 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | Cd14 |
| Gene ID | 60350 |
| Uniprot ID | CD14_RAT |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
| Protein Name | Monocyte Differentiation Antigen Cd14Myeloid Cell-Specific Leucine-Rich GlycoproteinCd Antigen Cd14 |
| Database Links | Reactome: R-RNO-140534Reactome: -RNO-166016Reactome: -RNO-166020Reactome: -RNO-166166Reactome: -RNO-2562578Reactome: -RNO-5686938Reactome: -RNO-6798695Reactome: -RNO-936964Reactome: -RNO-937041Reactome: -RNO-937072Reactome: -RNO-975163Reactome: -RNO-9824878 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedMembrane RaftGolgi ApparatusSolubleSecreted Forms Seem To ExistThey May Arise By Cleavage Of The Gpi Anchor |
| Alternative Antibody Pair Names | Monocyte Differentiation Antigen Cd14 antibody pairMyeloid Cell-Specific Leucine-Rich Glycoprotein antibody pairCd Antigen Cd14 antibody pairCd14 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat sCD14 (sCD14) Antibody Pair (STJAP00938)
This antibody pair detects rat soluble CD14 (sCD14), corresponding to UniProt entry Q63691. Reactivity has been confirmed for rat samples only.
The capture antibody is unconjugated and supplied at 0.5 mg/mL, while the detection antibody is biotinylated and supplied at 0.5 to 4 ug/mL. Both antibodies are raised in rabbit.
This pair is validated for use in sandwich ELISA with colorimetric detection. No other assay formats are specified for this product.
This pair has been confirmed for use with rat serum, plasma, and other biological fluids. Researchers should validate performance with any sample type not explicitly listed.
The recommended dilution range for the capture antibody is 1:1000 to 1:10000. Optimal working concentrations should be determined empirically for each laboratory setup.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.