This ENK Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma or other biological fluids..
Applications
ELISA
Reactivity
Rat
Sensitivity
25.13pg/mL
Detection Limit
93.7-6000pg/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Proenkephalin-A is cleaved by CTSL to generate Met-enkephalin. Met-enkephalin: Processed and degraded by ACE. Leu-enkephalin: Processed and degraded by ACE. Met-enkephalin-Arg-Gly-Leu: Probably cleaved by ACE. Met-enkephalin-Arg-Phe: Processed by ACE to generate Met-enkephalin in the nucleus accumbens of the brain. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Function
Met-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Leu-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Met-enkephalin-Arg-Phe: Met-enkephalin-Arg-Phe neuropeptide acts as a strong ligand of Mu-type opioid receptor OPRM1. Met-enkephalin-Arg-Phe-binding to OPRM1 in the nucleus accumbens of the brain increases activation of OPRM1, leading to long-term synaptic depression of glutamate release. PENK(114-133): Increases glutamate release in the striatum and decreases GABA concentration in the striatum. PENK(239-260): Increases glutamate release in the striatum.
