Rat DIO1 (Deiodinase, Iodothyronine, Type I) Sandwich ELISA Kit Ready-To-Use Kit (STJE0014813)

SPECIFICATIONS
STJE0014813
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General Information

Short DescriptionThis DIO1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates or other biological fluids..
ApplicationsELISA
ReactivityRat
Sensitivity0.059ng/mL
Detection Limit0.156-10ng/mL
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Storage InstructionThe whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time3 hrs

Target Information

Gene SymbolDio1
Gene ID25430
Uniprot IDIOD1_RAT
Immunogen RegionReady-To-Use
Sample Typetissue homogenates or other biological fluids.

Additional Info

Tissue Specificity Highly expressed in the liver, kidney and thyroid gland.
Post Translational Modifications
Function Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD). Catalyzes the deiodination of T4 to 3,3',5'-triiodothyronine (rT3), 3,5-diiodothyronine (3,5-T2) to 3-monoiodothyronine(3-T1) and 3,3'-diiodothyronine (3,3'-T2) to 3-T1 via inner-ring deiodination (IRD). Catalyzes the deiodination of rT3 to 3,3'-T2 via ORD. Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine, 3',5'-diiodothyronamine and 3,3'-diiodothyronamine as well as tyrosyl ring deiodinations of 3,5,3'-triiodothyronamine and 3,5-diiodothyronamine. Catalyzes the deiodination of T3 and 3,3',5-triiodo-L-thyronine sulfate via IRD. Catalyzes the deiodination of L-thyroxine sulfate via IRD and 3,3',5'-triiodo-L-thyronine sulfate via ORD.
Protein Name Type I Iodothyronine Deiodinase
5di
Dioi
Type 1 Di
Type-I 5'-Deiodinase
Database Links Reactome: R-RNO-350864
Cellular Localisation Cell Membrane
Single-Pass Type Iii Membrane Protein
Endoplasmic Reticulum Membrane
Basolateral Cell Membrane
Alternative ELISA Names Type I Iodothyronine Deiodinase ELISA kit
5di ELISA kit
Dioi ELISA kit
Type 1 Di ELISA kit
Type-I 5'-Deiodinase ELISA kit
Dio1 ELISA kit
Itdi1 ELISA kit
Txdi1 ELISA kit
output

Information sourced from Uniprot.org

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